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Plasmid#111576PurposeExpresses full length PARP1 with K to Q mutations in automodification domain in mammalian cellsDepositorInsertPARP1 (PARP1 Human)
UseTags3xFlagExpressionMammalianMutationlysine to glutamine mutation at the following ami…PromoterCMVAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV14/p14ARF
Plasmid#78760PurposeTo overexpress p14ARF in Mammalian CellsDepositorInsertARF (CDKN2A Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQFlag-USP11 CS puroR
Plasmid#46748PurposeRetroviral vector that expresses catalytically inactive form of Flag-tagged human USP11DepositorInsertUSP11 (USP11 Human)
UseRetroviralTagsFlagExpressionMammalianMutationC318S--catalytically inactivePromoterCMVAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC34
Plasmid#62331PurposesgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cellsDepositorInsertssgRNA + 2x MS2(wt+f6) binding module
MCP-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
TMED10-bio-His
Plasmid#51852PurposeExpresses full-length Transmembrane emp24 domain-containing protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED10 (TMED10 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)
Plasmid#214880PurposeLentiviral vector encoding RfxCas13d targeting CD46 guideDepositorInserthU6-crCD46-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSF4 CMV 5TOP intron renilla TRICK CTE polyA
Plasmid#64544Purpose5′ TOP TRICK reporter mRNADepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, 5' TOP (5′ terminal oligo…ExpressionMammalianMutationPromoterTetracycline-inducible promoter and 5′ UTR of hum…Available sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC78
Plasmid#62339PurposesgRNA + 1x COM with COM-KRAB effector for mammalian cellsDepositorInsertssgRNA + 1x COM binding module
COM-KRAB
UseLentiviralTagsKRABExpressionMammalianMutationTargets sv40 promoter, sequence: CATACTTCTGCCTGCT…PromoterCMV and U6Available sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
EE-LRRK2
Plasmid#193583PurposeExpresses full-length LRRK2 with FYVE-CC2 domains from HRS and 3xFLAG on the N-terminusDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
UseTagsFYVE-CC2 and 3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIR-21 Reporter
Plasmid#71870PurposeMammalian expression vector for the analysis of miR-21 activityDepositorInsertReverse complementary sequence of miR-21
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)
Plasmid#214877PurposeLentiviral vector encoding RfxCas13d targeting LPT guide arrayDepositorInserthU6-crLPT-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4EBP1 F113A
Plasmid#81122Purposeexpresses a constitutively active mutant of 4E-BP1 under the CAG promoterDepositorInsert4EBP1 F113A (Eif4ebp1 Mouse)
UseTagsExpressionMammalianMutationF113A mutationPromoterCAGAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-CMV HA ALAS-1
Plasmid#22746DepositorInsertALAS-1 (Alas1 Mouse)
UseAdenoviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
PDPN-bio-His
Plasmid#52313PurposeExpresses full-length Podoplanin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPDPN (PDPN Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
UseTagsExpressionBacterialMutationPromotertrcpAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_Ruby
Plasmid#110164PurposeCas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNADepositorInserthSpCas9
UseCRISPRTags3xFLAG and Ruby2ExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DCX-2A-mCherry
Plasmid#118290PurposeExpresses human DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.DepositorInsertdoublecortin (DCX Human)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-1 shRev-Erbalpha
Plasmid#22747DepositorInsertshRev-Erbalpha (Nr1d1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJZC33
Plasmid#62330PurposesgRNA + 2x MS2 with MCP-VP64 effector for mammalian cellsDepositorInsertssgRNA + 2x MS2 binding module
MCP-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUT8-Dox
Plasmid#124631PurposeDoxycycline-inducible FUT8 expression in mammalian cellsDepositorInsertsAlpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only