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5HT6-YFP-Inp54p(D281A)
Plasmid#96813Purposephosphatase-dead control for 5HT6-YFP-Inp54pDepositorInsert5HT6-YFP-Inp54p(D281A)
UseTagsYFPExpressionMammalianMutationD281A inactivates phosphatase activityPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-p3E-HyPer7
Plasmid#194289PurposeMultisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQVDepositorInsertHyper7
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PI4KAC1001
Plasmid#139311PurposeRecruitable PI4KA catalytic fragmentDepositorInsertmCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
UseTagsExpressionMammalianMutationG1986DPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
iRFP-Sec61beta
Plasmid#108125PurposeER markerDepositorInsertiRFP:SEC61B (SEC61B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher
Plasmid#223633PurposeProtein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-hs_ICAM_short (JDW 1046)
Plasmid#224480PurposeGateway compatible 5' entry clone containing the Human minimal ICAM2 promoterDepositorInsertmin-ICAM2 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform1
Plasmid#70173PurposeMammalian expression of hsOPA1 isoform 1DepositorInserthsOPA1 isoform 1 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA E2F2
Plasmid#24226PurposeMammalian expression of human E2F2 with HA tagDepositorInsertE2F2 (E2F2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDULE-ABK
Plasmid#49086PurposeA pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experimentsDepositorInsertstRNA synthetase
tRNA for a photocrosslinking amino acid
UseTagsExpressionBacterialMutationL274M, C313A, Y349FPromoterGlnRS and lppAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
AA302
Plasmid#216052PurposeFragmid fragment: (C' terminus) Nucleoplasmin NLSDepositorHas ServiceCloning Grade DNAInsertNpNLS_v1.2; Stop; polyA_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPML1 (GB0045)
Plasmid#68164PurposeProvides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid partDepositorInsertAtML1 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Sox10
Plasmid#46536PurposeA lentiviral vector for the expression of mouse Sox10 under doxycyline control.DepositorInsertSox10 (Sox10 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Lyn-dInPAkt
Plasmid#181834PurposeGenetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).DepositorInsertLyn-dInPAkt
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir1-LNS2
Plasmid#220083Purposehigh affinity PA biosensor with green fluorophoreDepositorInsertPITPNM3 (PITPNM3 Human)
UseTagsNeonGreen-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EKAR4
Plasmid#174437PurposeImproved cyan/yellow FRET-based ERK kinase activity reporter.DepositorInsertEKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform7
Plasmid#70179PurposeMammalian expression of hsOPA1 isoform 7DepositorInserthsOPA1 isoform 7 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-Rbfox3
Plasmid#213727PurposeMammalian cell expression vector for overexpression of Rbfox3 in motor neurons.DepositorInsertRbfox3 (Rbfox3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG-RGG
Plasmid#124938PurposeExpression of RGG-RGG-RGGDepositorInsertRGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Akt1(WT)
Plasmid#86631PurposeExpresses mCherry-tagged human Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xFLAG-Pontin
Plasmid#51635PurposeMammalian expression of 3xFLAG tagged pontin, N-terminal.DepositorInsertPontin (RUVBL1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pJL1-sfGFP-DQNAT
Plasmid#198210PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_DQNAT
UseTags6x His Tag and DQNAT glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPI/hPLK2WT/Neo
Plasmid#35385DepositorInserthPLK2 (PLK2 Human)
UseLentiviralTagsEMCV IRES-Neomycin cassette and V5 epitope and Po…ExpressionMammalianMutationPromoterEF1-alphaAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCit-4aa-SNAP25B
Plasmid#53235PurposeFluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
UseTags4aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-NFLAG TRF1 deltaA deltaM
Plasmid#16065DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMutationDeleted acidic domain (aa1-66) and myb domain (aa…PromoterAvailable sinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag
Plasmid#179715PurposeLentiviral expression of mouse Syt7 with C terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-SINTBAD
Plasmid#216840PurposeStably express FKBP-GFP-tagged SINTBAD for CID induced mitophagyDepositorInsertSINTBAD (TBKBP1 Human)
UseLentiviralTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CRY2-mCh-superPLDx10
Plasmid#188986PurposeA lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)DepositorInsertPLD
UseTagsCRY2 and mCherryExpressionMammalianMutationF163L, K249R, G328S, G381V, G429D, D480GPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB-Dcp1b
Plasmid#153979PurposeDimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1bDepositorInsertmRNA decapping enzyme 1b (DCP1B Human)
UseTagspAcGFP-C1ExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
mRFP-LAP2β
Plasmid#228029Purposelentiviral construct for mRFP-LAP2β expressionDepositorInsertLAP2b (Tmpo Rat)
UseLentiviralTagsmRFPExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir2-LNS2
Plasmid#220086PurposeC-terminal PA binding domain of Nir2DepositorInsertPITPNM1 (PITPNM1 Human)
UseTagsNeonGreen-GGSGGMExpressionMammalianMutationamino acids 896-1216PromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CRY2-mCh-superPLDx30
Plasmid#188988PurposeA lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)DepositorInsertPLD
UseTagsCRY2 and mCherryExpressionMammalianMutationK109R, P245A, G328S, G381V, G429DPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pPBpuro-miRFP670-HRasCT
Plasmid#176030PurposeEncoding membrane-targeted miRFP670DepositorInsertMembrane-targeted miRFP670
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-Rbfox1
Plasmid#213725PurposeMammalian cell expression vector for overexpression of Rbfox1 in motor neurons.DepositorInsertRbfox1 (Rbfox1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform5
Plasmid#70177PurposeMammalian expression of hsOPA1 isoform 5DepositorInserthsOPA1 isoform 5 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dPlcR
Plasmid#181832PurposeGenetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).DepositorInsertLyn-PlcR
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2
Plasmid#25031DepositorInsertDCP2 (DCP2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-NCAM-1
Plasmid#205199PurposeThis vector encodes of the synCAM (NCAM1) and GFP nanobody LAG16DepositorInsertsynCAM NCAM 1 and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
J23100_GB
Plasmid#65983PurposeMoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
μ2-HA-D176A
Plasmid#32754DepositorInsertμ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
UseTagsHAExpressionMammalianMutationD176APromoterCMVAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-p62
Plasmid#199780PurposeUsed for the expression of mCherry labelled p62 (SMC Internal No. 391).DepositorInsertp62 (NUP62 Human)
UseTags6x His, TEV, mCherryExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-fibcon-iCAM1
Plasmid#205212PurposeThis vector encodes of the synCAM (fibcon - iCAM1) and GFP nanobody LAG16DepositorInsertsynCAM fibcon - iCAM1 and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP-PML-IV-mSIM-C3
Plasmid#134529PurposeExpresses Cherry-PML-IV fusion protein with a mutation in its SIMDepositorInsertPML-IV-mSIM
UseTagsYFPExpressionMammalianMutationSIM (VVVI) 556-559 aa changed to VGGGPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas13d-T2A-Citrine
Plasmid#224565PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.DepositorInsertCas13d-T2A-Citrine
UseCRISPRTagsT2A-CitrineExpressionMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
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OPA1-isoform2
Plasmid#70174PurposeMammalian expression of hsOPA1 isoform 2DepositorInserthsOPA1 isoform 2 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyTag003-MBP
Plasmid#184227PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.DepositorInsertAviTag-SpyTag003-MBP
UseTagsAviTag, His6, SpyTag003, and Thrombin cleavage si…ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only
