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pClathrin-miRFP670nano3

Plasmid

#184667

Purpose

Clathrin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-clathrin light chain

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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Tailless VinculinTS

Plasmid

#26020

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

tailless vinculinTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pMyosin-miRFP670nano3

Plasmid

#184668

Purpose

LC-Myosin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-LC-Myosin

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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beta-arrestin2 mYFP

Plasmid

#36917

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin2 (Arrb2 Rat)

Use

Tags

mYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MYC-Sumo3 WT

Plasmid

#48964

Purpose

Expresses MYC-Sumo3 Wild Type in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO3 (SUMO3 Human)

Use

Tags

MYC (EQKLISEED)

Expression

Mammalian

Mutation

Promoter

T7

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-KIRIN1-GR

Plasmid

#107418

Purpose

Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1-GR

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1-GR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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FlagETR3 (CELF2)

Plasmid

#96900

Purpose

expression of Flag-tagged human CELF2 (aka CUGBP2)

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

human CELF2 cDNA (CELF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1-Nir3-LNS2

Plasmid

#220087

Purpose

C-terminal PA binding domain of Nir3

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

PITPNM2 (PITPNM2 Human)

Use

Tags

mEGFP-GGGGSHM

Expression

Mammalian

Mutation

amino acids 925-1209

Promoter

CMV

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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LgBit-NbV5

Plasmid

#201476

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

LgBit-NbV5

Use

Tags

LgBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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beta-arrestin1 mYFP

Plasmid

#36916

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin1 (Arrb1 Rat)

Use

Tags

mYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos WT

Plasmid

#8966

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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pCDNA 3.1 Flag-rat-PTP1B

Plasmid

#35988

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-NBid-PhoCl2c-CBid

Plasmid

#164051

Purpose

Optogenetic manipulation of cell apoptosis using PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NBid-PhoCl2c-CBid

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p3s-Sniper-Cas9

Plasmid

#113912

Purpose

Expresses Sniper-Cas9 with CMV promoter

Depositor

Jungjoon Lee

Article

Lee et al Nat Commun. 2018 Aug 6;9(1):3048. doi: 1

Insert

Sniper-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEV53D

Plasmid

#44168

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Insert

Equine infectious anemia virus (EIAV) gag-pol,rev, and S2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

A131T mutation in gag; Deletion of viral LTR sequ…

Promoter

CMV

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pFH2.12_GagZip-MCP-P2A-eGFP

Plasmid

#205527

Purpose

Express HIV GagZip-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

GagZip-MCP

Use

Tags

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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MaLionB

Plasmid

#113910

Purpose

Blue ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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MmXYRS-4xM15

Plasmid

#155343

Purpose

Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.

Depositor

Irene Coin

Article

Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

Insert

Humanized MmXYRS/tRNAM15

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/U6

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-CalfluxCTN

Plasmid

#190068

Purpose

Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

Depositor

Carl Johnson

Article

Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

Insert

CalfluxCTN

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 WT

Plasmid

#8974

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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