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pCAG-MBP-Foldon-ATG9 (723-839)

Plasmid

#188088

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (723-839)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9 (801-839)

Plasmid

#188093

Purpose

To make monomeric ATG9 C-terminal tail for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (801-839)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (828-839)

Plasmid

#188090

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (828-839)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)

Plasmid

#188075

Purpose

To make ATG101/ATG13 HORMA complex for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTBL405 CHYRON1 integration construct

Plasmid

#126442

Purpose

To integrate the CHYRON1 locus at HEK293site3.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6-CHYRON1 hgRNA
pCMV-puro

Use

Tags

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoBa

Plasmid

#121897

Purpose

Plasmid for making zebrafish apoBa in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoBa (apoba Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-N3FLAG-TAF12(50-130)

Plasmid

#105595

Purpose

retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

truncation fragments containing aa (50-130), and …

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9-KRAB-MeCP2

Plasmid

#110824

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

Depositor

George Church, Alejandro Chavez

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Use

Tags

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASHΔPPPL

Plasmid

#129280

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

Depositor

Daniel Conway

Article

Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Expression

Mutation

the last 4 amino acids (PPPL) of the KASH domain …

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-XL-s2193

Plasmid

#166749

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-XL (BCL2L1 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

Human ferritin

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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DENV4 Rluc Reporter GND mutant Replicon

Plasmid

#118586

Purpose

For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

Depositor

Radhakrishnan Padmanabhan

Article

Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

Insert

DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.

Use

Yeast-e. col shuttle vector prs424

Tags

Expression

Mutation

The conserved GDD in the NS5 polymerase gene muta…

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-PAmCherry-mSNCA-NE

Plasmid

#102364

Purpose

Lentiviral overexpression of PA-mCherry synuclein alpha fusion

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Inserts

PA-mCherry
synuclein alpha (Snca Mouse)

Use

Lentiviral

Tags

NE

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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BCR/ABL P190 transgenic construct

Plasmid

#38185

Depositor

Nora Heisterkamp

Article

Heisterkamp et al Nature. 1990 Mar 15;344(6263):251-3.

Insert

BCR/ABL P190 (ABL1, BCR Human)

Use

Construct for making transgenic mice

Tags

Expression

Mutation

Promoter

truncated mouse MT promoter

Available since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pcdna6.2- myc ULK1 k46I

Plasmid

#27630

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Promoter

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pegRNA-GFP>BFP_Y66H-NGG

Plasmid

#185480

Purpose

pegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

GFP>BFP_Y66H-pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-W-FUGW

Plasmid

#166751

Purpose

Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Bcl-W (Bcl2l2 Mouse)

Use

Lentiviral

Tags

mCerulean3

Expression

Mutation

Promoter

human ubiquitin C

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pKIF1C-GFP

Plasmid

#130977

Purpose

Expression of KIF1C-GFP in mammalian cells.

Depositor

Anne Straube

Article

Efimova et al J Cell Sci. 2014 Dec 15;127(Pt 24):5179-

Insert

KIF1C-GFP (KIF1C Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

5 silent mutations that make this construct RNAi …

Promoter

CMV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis

Plasmid

#157534

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3E (SEMA3E Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA3F-Fc(DAPA)-AviTag-6xHis

Plasmid

#156977

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3F (SEMA3F Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

Plasmid

#102421

Purpose

Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector

Depositor

Kian Peng Koh

Article

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Insert

Tet1 (Tet1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Modified at the Dharmacon SMARTpool siRNA #2 targ…

Promoter

TetO-CMV

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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