-
Plasmid#159878PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG2 - 250bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2369 - intron GG1 - 250bp
Plasmid#159877PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG1 - 250bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2370 - intron GG3 - 250bp
Plasmid#159879PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG3 - 250bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dimer cleaved
Plasmid#198199PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavageDepositorInsertmVenus-T2A-mTurquoise2
UseTagsExpressionMammalianMutationA206K bothPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-Renilla
Plasmid#79496PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDSDepositorInsertRenilla Luciferase
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-Firefly
Plasmid#79495PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDSDepositorInsertFirefly Luciferase
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-sGFP
Plasmid#79508PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDSDepositorInsertsuperGFP
UseMultisite gateway entry cloneTags6xHisExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPPC009.106
Plasmid#171142PurposeExpression of J106 scRNA on pBBR1-KmR plasmidDepositorInsertJ106 scRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12D/C185S
Plasmid#83165PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D/C185S mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12D/C185SPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-WNT3A
Plasmid#79501PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDSDepositorInsertWNT3A (WNT3A Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMLS538
Plasmid#188881PurposeGateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2)DepositorInsertCas9
UseGateway entry vectorTagsExpressionMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3
Plasmid#73542PurposeExpression of sgRNA targeting LINE-1 and TagBFPDepositorInsertLINE-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1
Plasmid#73543PurposeExpression of sgRNA targeting LINE-1 and TagBFPDepositorInsertLINE-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2365 - Intron GG1 - 250bp no PATC
Plasmid#159883PurposeGolden Gate compatible intron with no PATCs for negative controlsDepositorInsertIntron GG1 - 250bp no PATC
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2345 - Intron GG2 - 250bp no PATC
Plasmid#159884PurposeGolden Gate compatible intron with no PATCs for negative controlsDepositorInsertIntron GG2 - 250bp no PATC
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2366 - intron GG3 - 250bp no PATC
Plasmid#159885PurposeGolden Gate compatible intron with no PATCs for negative controlsDepositorInsertintron GG3 - 250bp no PATC
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pATF1-donor
Plasmid#112328PurposeCRISPR donor plasmid to tag human transcription factor ATF1 with GFPDepositorInsertATF1 homology arms flanking EGFP-IRES-Neo cassette (ATF1 Human)
UseCRISPRTagsExpressionMutationhg38:12:50819876:T>A, hg38:12:50819888:T>C,…PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CMV.Pro
Plasmid#79486PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoterDepositorInsertCMV Promoter
UseMultisite gateway entry cloneTagsExpressionMutationPromoterCMV PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA
Plasmid#129513PurposeExpresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cellsDepositorInsertUBE2QL1 C88S (UBE2QL1 Human)
UseTags2xStrep/HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159856PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only