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Plasmid#183359PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1 (CASP1 Human)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ3M-Mst1 K59R
Plasmid#12204DepositorUseTagsMycExpressionMammalianMutationCatalytically inactive: K59RPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
Dynamitin-GFP
Plasmid#51419Purposeexpression of GFP-tagged wild-type dynamitin p50 in mammalian cellsDepositorInsertDynamitin (DCTN2 Chicken)
UseTagsGFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lmajor_gp63_10_0460_WT
Plasmid#171640PurposeExpression of wildtype L. major glcyoprotein-63 from chromosome 10 in mammalian cellsDepositorInsertLmjF.10.0460
UseTagsMyc-HisExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg30-PDGFR_EF1a_mCherry
Plasmid#186309PurposePlasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg30-PDGFR
mCherry
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lmajor_gp63_10_0460_E265A
Plasmid#171641PurposeExpression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cellsDepositorInsertLmjF.10.0460 E265A
UseTagsMyc-HisExpressionMammalianMutationE265APromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriExEKAR2G2
Plasmid#40175DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MT-mitfa
Plasmid#81231Purposeexpression of zebrafish mitfaDepositorInsertmitfa (mitfa Zebrafish)
UseZebrafish expressionTags6 myc tagsExpressionMammalianMutationcontains amino acids 5-412PromotersCMV IE94 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_175_Venus_173
Plasmid#39830DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD.K/W
Plasmid#10809DepositorInsertPKD (PRKD1 Human)
UseTagsHA and Myc (inserted between amino acids 33&3…ExpressionMammalianMutationKinase dead mutant: K612W (Lys 612 to Trp) (bp183…PromoterCMVAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDBEcR/Bla
Plasmid#201556PurposePlasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.DepositorInsertDBEcR
UseUnspecified; Sleeping beauty transposonTagsMycExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
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NbV5-Rluc8
Plasmid#201480PurposeBRET detection of V5-tagged proteinDepositorInsertRluc8-NbV5
UseTagsRluc8ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01(ΔL1)
Plasmid#80049PurposeExpression of EPN-01 mutated in TSG101 binding site.DepositorInsertEPN-01(deltaPTAP)
UseTagsMycExpressionMammalianMutationPTAP10AAAAPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
NbV5-GFP2
Plasmid#201477PurposeBRET2 detection of V5-tagged proteinDepositorInsertNbV5-GFP2
UseTagsGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-deltaA1-Lock-VWF
Plasmid#124824PurposeExpresses multimeric human VWF protein without A1 domain and Lock A2 domain. This protein has C-terminal c-Myc tag.DepositorInsertVWF without A1 domain and Lock A2 domain (VWF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-psba1-TetR-PL22-dCas9-SpR
Plasmid#73223PurposeContains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.DepositorInsertdCas9 from S. pyogenes
UseTagsc-mycExpressionBacterialMutationSilent mutation at bp 1341 A->C to remove an E…PromoterPL22Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
NbALFA-TEV219
Plasmid#201487PurposeTANGO detection of V5-tagged proteinDepositorInsertNbALFA-TEV219
UseTagsTEV219ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
NbV5-TEV219
Plasmid#201481PurposeTANGO detection of V5-tagged proteinDepositorInsertNbV5-TEV219
UseTagsTEV219ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1 H83L H86L
Plasmid#12211DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationPBD (p21-binding domain) mutant: H83L, H86L (see …PromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only