-
Plasmid#148551PurposeMammalian Expression of Hs4EHP-S99NDepositorInsertHs4EHP-S99N (EIF4E2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TAPtagN-PrP-SBP-HsAGO2_AC
Plasmid#148440PurposeMammalian Expression of HsAGO2DepositorInsertHsAGO2 (AGO2 Human)
UseTagsExpressionMammalianMutationone silent mutation C840Tand sequence differs in …PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres_AC
Plasmid#148442PurposeMammalian Expression of HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAresDepositorInsertHsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP3-L61AV67A_Z
Plasmid#148147PurposeMammalian Expression of Hs4EBP3-L61AV67ADepositorInsertHs4EBP3-L61AV67A (EIF4EBP3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP3-Y40AL45A_Z
Plasmid#148148PurposeMammalian Expression of Hs4EBP3-Y40AL45ADepositorInsertHs4EBP3-Y40AL45A (EIF4EBP3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP3-YLLVtoAAAA_Z
Plasmid#148149PurposeMammalian Expression of Hs4EBP3-YLLVtoAAAADepositorInsertHs4EBP3-YLLVtoAAAA (EIF4EBP3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP1-L75AV81A_Z
Plasmid#148144PurposeMammalian Expression of Hs4EBP1-L75AV81ADepositorInsertHs4EBP1-L75AV81A (EIF4EBP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP1-Y54AL59A_Z
Plasmid#148145PurposeMammalian Expression of Hs4EBP1-Y54AL59ADepositorInsertHs4EBP1-Y54AL59A (EIF4EBP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP1-YLLVtoAAAA_Z
Plasmid#148146PurposeMammalian Expression of Hs4EBP1-YLLVtoAAAADepositorInsertHs4EBP1-YLLVtoAAAA (EIF4EBP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9-shRNAres1_W
Plasmid#147904PurposeMammalian Expression of HsNot9-shRNAres1DepositorInsertHsNot9-shRNAres1 (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9-shRNAres2-H58AF60AA64YV71Y_W
Plasmid#147906PurposeMammalian Expression of HsNot9-shRNAres2-H58AF60AA64YV71YDepositorInsertHsNot9-shRNAres2-H58AF60AA64YV71Y (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-GFP-AnillinA740D, E758K
Plasmid#187274PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758KDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsEGFPExpressionMutationAnillin A740D, E758KPromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-mDia1-GBD (1-305 aa) in pEGFPN1
Plasmid#187280PurposeExpress EGFP-mDia1 GTPase-binding domainDepositorInsertmDia1 (Diaph1 Mouse)
UseTagsEGFPExpressionMammalianMutationGBD (1-305aa)PromoterCMVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
IL2-mCherry-rGBD in pmCherryN1
Plasmid#187286PurposeExpress IL2-Rhotekin GTPase-binding domain-mCherryDepositorUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-NLSmCherryNLS Clover-HDHB
Plasmid#190223PurposeFluorescent cell cycle reporterDepositorInsertsUseTransposonTagsClover fluorescent proteinExpressionMammalianMutationAmino Acids 994-1087PromoterEF1a and RPBSAAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3-JD
Plasmid#184247PurposeRecombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)DepositorInsertAtaxin-3 Josephin domain (ATXN3 Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationPremature stop codon V183XPromoterT7 promoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3-D1
Plasmid#184246PurposeRecombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertAtaxin-3 Josephin domain and UIM1 (ATXN3 Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationPremature stop codon N263XPromoterT7 promoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4 TAPBPR-TM-TN6
Plasmid#178650PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)DepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationE205K, R207E, Q209S, Q272S, switched transmembran…PromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMX-SHP1C/S-ires-mCD8
Plasmid#183719PurposeExpresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.DepositorInsertSHP-1
UseRetroviralTagsmycExpressionMutationC453SPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J
Plasmid#146702PurposeInsect Expression of DmNot1_1710-2505DepositorInsertDmNot1_1710-2505 (Not1 Fly)
UseTagsExpressionInsectMutationtwo non silent mutations (A1747T, G1999S) and one…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AF
Plasmid#148686PurposeBacterial Expression of HsGIGYF2_35-105-E46AE47FN91ADepositorInsertHsGIGYF2_35-105-E46AE47FN91A (GIGYF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1_1-177-YYMLtoAAAA_AE
Plasmid#148575PurposeMammalian Expression of HsGIGYF1_1-177-YYMLtoAAAADepositorInsertHsGIGYF1_1-177-YYMLtoAAAA (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-CvH-Hs4EHP_52-234opt_AE
Plasmid#148564PurposeBacterial Expression of Hs4EHP_52-234optDepositorInsertHs4EHP_52-234opt (EIF4E2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9-shRNAres2_W
Plasmid#147905PurposeMammalian Expression of HsNot9-shRNAres2DepositorInsertHsNot9-shRNAres2 (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1-K1537SG1562EQ1658A-dsRNAres_W
Plasmid#147860PurposeInsect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAresDepositorInsertDmNot1-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_812-1945-dsRNAres_W
Plasmid#147845PurposeInsect Expression of DmNot1_812-1945-dsRNAresDepositorInsertDmNot1_812-1945-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsNot1iso1_1356-1581_V
Plasmid#147779PurposeBacterial Expression of HsNot1iso1_1356-1581DepositorInsertHsNot1iso1_1356-1581 (CNOT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC
Plasmid#148420PurposeMammalian Expression of HsRck_296-472-10xmutDepositorInsertHsRck_296-472-10xmut (DDX6 Human)
UseTagsExpressionMammalianMutationQ309A, H312A, T316A, R320A, Q322A, N324A, R335A, …PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
Plasmid#179434PurposeDual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-HS4-mch (SH)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL006_phiC31_neo-5xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry
Plasmid#179425PurposeDual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-(nospacer)-mch
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo2 mycBioID-Tollip M240A,F241A
Plasmid#178055Purposemyc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.DepositorInsertToll-Interacting Protein (TOLLIP Human)
UseTagsmycBioIDExpressionMammalianMutationChanged Methionine 240 to Alanine and changed Phe…PromoterCMVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-Citrine-ADPG2
Plasmid#170725PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)DepositorInsertARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
UseGateway donor vector / entry cloneTagsCitrine tag (714bp)ExpressionMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -2
Plasmid#173891PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔEC gRNA resistant
Plasmid#163413PurposeExpresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe extracellular domain (27-150 aa) is deleted. …PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔTail gRNA resistant
Plasmid#163412PurposeExpresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe C-terminal tail domain (530-1038 aa) is delet…PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔKinase gRNA resistant
Plasmid#163411PurposeExpresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe kinase domain (202-500 aa) is deleted. gRNA-t…PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlinkG2-PTPIP51(236-470)
Plasmid#170534PurposeExpresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsGST tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(36-235)
Plasmid#170533PurposeExpresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsHis tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFASTbac-HisGST-PTPIP51(36-470)
Plasmid#170531PurposeExpresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9DepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 )
UseTagsGST tag and His tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(236-470)
Plasmid#170530PurposeExpresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)
UseTagsHis tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorInsertssrp1 (Ssrp Fly)
UseTags6xHis and V5ExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TUBG2
Plasmid#171966PurposeExpression in cell lines of the TUBG2 geneDepositorInsertTUBG2 (TUBG2 Human)
UseTagsExpressionMammalianMutationRemoval of the internal TUBG2-EcoRI site by chang…PromoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G
Plasmid#157982PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (Tetra-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV126
Plasmid#124250PurposeIn vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV127
Plasmid#124251PurposeIn vitro transcription plasmid for T7 transcription of Her2 ITD sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV128
Plasmid#124252PurposeIn vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV129
Plasmid#124253PurposeIn vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templateTagsExpressionMutationPromoterT7Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)
Plasmid#166855PurposeExpresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeastDepositorInsertAtg13[571–700] (ATG13 Budding Yeast)
UseTags6xHisExpressionYeastMutationK683E, H685E, K686EPromoterpCuAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pCMV_eA3A T31A-nCas9
Plasmid#163538PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)eA3A T31APromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only