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MBP-3C-hTspan12LEL(118-218)_pAcGP67a

Plasmid

#216383

Purpose

Baculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM014_P2_pGPM1

Plasmid

#216439

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter gpm1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK201

Plasmid

#207131

Purpose

Type 2 ptac promoter part

Depositor

Nicholas Shikuma

Article

Alker et al mBio. 2023 Aug 2:e0150223. doi: 10.1128/

Insert

ptac promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM025_P2_pTUP11

Plasmid

#216450

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter tup11

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM023_P2_pAPL4

Plasmid

#216448

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter apl4

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM021_P2_pTUB1

Plasmid

#216446

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter tub1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM018_P2_pRPL701

Plasmid

#216443

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rpl701

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM017_P2_pTIF51

Plasmid

#216442

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter tif51

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM016_P2_pRPL2501

Plasmid

#216441

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rpl2501

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pT7RNAP-T15

Plasmid

#199991

Purpose

T7 RNAP circuit variant T15 on miniRK2 plasmid

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-blast

Plasmid

#210221

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRC043

Plasmid

#206799

Purpose

J23107.SoxS-GID1

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

SoxS-GID1

Use

Tags

Expression

Mutation

Promoter

J23107

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRC025

Plasmid

#206796

Purpose

J23107.MCP-ABI

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

MCP-ABI

Use

Tags

Expression

Mutation

Promoter

J23107

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRC011

Plasmid

#206794

Purpose

J23107.MCP-SYNZIP6

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

MCP-SYNZIP

Use

Tags

Expression

Mutation

Promoter

J23107

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pPath-BGC08-pT7native

Plasmid

#200005

Purpose

BGC08 pathway. pT7-native CDS sequence

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRC012

Plasmid

#206795

Purpose

J23107.SoxS-SYNZIP5

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

SoxS-SYNZIP

Use

Tags

Expression

Mutation

Promoter

J23107

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pRC027

Plasmid

#206797

Purpose

J23107.SoxS-PYL1

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

SoxS-PYL1

Use

Tags

Expression

Mutation

Promoter

J23107

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRC029

Plasmid

#206793

Purpose

J23107.MCP-SoxS

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

MCP-SoxS

Use

Tags

Expression

Mutation

Promoter

J23107

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRC042

Plasmid

#206798

Purpose

J23107.MCP-GAI

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

MCP-GAI

Use

Tags

Expression

Mutation

Promoter

J23107

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald

Plasmid

#201187

Purpose

Lentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Bart Nieuwenhuis

Inserts

PTPN2, transcript variant 2 (PTPN2 Human)
Emerald

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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