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Plasmid#52067PurposeExpresses full-length Gelsolin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertGSN (GSN Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pLVX neo PFKP-GFP K281R
Plasmid#138288PurposeMammalian Expression, Lentiviral. Expression of human phosphofructokinase platelet isoform PFKP-GFP fusion protein with K281R mutationDepositorInsertPFKP (PFKP Human)
UseLentiviralTagsGFPExpressionMammalianMutationK281RPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc
Plasmid#21850DepositorInsertATF4 (Atf4 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationATF4 5' UTR and AUG fused to Luciferase driv…PromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_MAPT:EGFP
Plasmid#92196PurposeLentiviral overexpression vector to make stable bicistronic cell line for Tau screenDepositorInsertDsRed-IRES-MAPT-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP (empty vector)
Plasmid#172604PurposeEmpty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cellsDepositorTypeEmpty backboneUseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)- e18 LOF
Plasmid#131729PurposeExpresses a loss of function mutant of an engineered variant of RAD18 ("e18")DepositorInserte18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7501 pHR (hU6-crNT-EFS-PuroR-WPRE)
Plasmid#214876PurposeLentiviral vector encoding RfxCas13d nontargeting control guideDepositorInserthU6-crNT-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJZC74
Plasmid#62342PurposesgRNA + 1x COM with COM-KRAB effector for mammalian cellsDepositorInsertssgRNA + 1x COM binding module
COM-KRAB
UseLentiviralTagsKRABExpressionMammalianMutationTargets sv40 promoter, sequence: GAATAGCTCAGAGGCC…PromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pG2J
Plasmid#102882PurposeExpresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertsOsTIR1
mGFP-AID
UseTagsAID(71-114)ExpressionYeastMutationPromoterADH1 and TEF1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD47-bio-His
Plasmid#53150PurposeExpresses full-length CD47 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD47 (CD47 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-gamma-tubulin
Plasmid#87956PurposeExpresses human gamma-tubulin with a GFP-tag on the C-terminal of the proteinDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsGFPExpressionMammalianMutationThe protein is a sh-gamma-tubulin resistant gene …PromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJZC116
Plasmid#62344PurposesgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFPDepositorInsertssgRNA + 2x MS2 (wt+f6) binding module
MCP-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGCPromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCMV6-Neo-ISG15-LRAA
Plasmid#80405Purposeoverexpression of nonconjugative ISG15DepositorInsertISG15 (ISG15 Human)
UseTagsExpressionMammalianMutationnonconjugative ISG15, LRAAPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92228PurposeExpression of zmADH using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
TPC1-mCherry
Plasmid#135182PurposeExpresses TPC1 with C-terminus mCherry tag in mammalian cellsDepositorInsertTPC1 (Tpcn1 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5-YFP-NLS-mut
Plasmid#120832PurposeEctopic expression of ZIKV proteinDepositorInsertZIKV NS5-eYFP "NLS-mut"
UseTagseYFP-V5ExpressionMammalianMutationR372A/Q373A/K387A/H388A/K389APromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only