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Plasmid#67730PurposeExpresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged histidine 303 to alaninePromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_D230A
Plasmid#67725PurposeExpresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged aspartate 230 to alaninePromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_N191A
Plasmid#67724PurposeExpresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged asparagine 191 to alaninePromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_K295A
Plasmid#67726PurposeExpresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationChanged lysine 295 to alaninePromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAW-GFP-2xmiR-34 (pAW-AS-25)
Plasmid#40833PurposemiRNA sensor used in Drosophila cell linesDepositorInsertTwo target sites perfectly complementary to miR-34
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYes2-AtPCS1
Plasmid#49766PurposeExpresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces CerevisiaeDepositorInsertAtPCS1 (PCS1 Mustard Weed)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNM3746
Plasmid#154109PurposeloxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'
UseCre/Lox; RmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDHt-KS
Plasmid#63616PurposeThe multiple cloning site of pGreenII0000 cloned into binary vector pDHtDepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike2
Plasmid#136949PurposePlasmid for the In Vitro transcription of DiySpike2DepositorInsertDiySpike2
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
AU1-TTC30A1-pcDNA3
Plasmid#47323PurposeExpresses AU1 epitope-tagged TTC30A1 in mammalian cellsDepositorInsertTTC30A1 (Ttc30a1 Mouse)
UseTagsAU1 epitopeExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-SELF
Plasmid#158770PurposeExpression of synaptophysin 1 variant with self-cleaving c-terminal tail.DepositorInsertSynaptophysin 1-NS3/4a (Syt1 Mouse)
UseLentiviralTagsExpressionMutationFlexible linkers, ADVVCC/QMSY cleavage sitePromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
TLS 3: TLS.pRSET.C
Plasmid#21829DepositorInsertTLS (FUS Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWRKY70::LUC
Plasmid#141171PurposeLuminescent reporter for salicylic acid signalingDepositorInsertAtWRKY70 promoter
UseLuciferaseTagsExpressionPlantMutationPromoterAtWRKY70, AT3G56400Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW-EGFP-2xmiR-1 (pAW-AS-21)
Plasmid#40832PurposemiRNA sensor used in Drosophila cell linesDepositorInsertTwo target sites perfectly complementary to miR-1
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSP571
Plasmid#139498PurposeCRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNADepositorInsertpGPD Cas9 / sgRNA (STL162)
UseTagsExpressionYeastMutationWTPromoterpGPDAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
XE40 pSP64T-PABP (Clone 63)
Plasmid#17019DepositorInsertPoly(A) binding protein (pabpc1.S Frog)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAG500-FKBP-N-frFAST
Plasmid#160060PurposeExpresses FKBP-N-frFAST in mammalian cellsDepositorInsertFKBP-N-frFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTEI134
Plasmid#126071PurposeVector for expression of plastid targeted YFP1-10 (T203Y) in plant cellDepositorInsertChloroplastic FNR transit peptide
UseBinaryTagsYFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI135
Plasmid#126073PurposeVector for expression of mitochondria targeted YFP1-10 (T203Y) in plant cellDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsYFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI165
Plasmid#126074PurposeVector for expression of plastid targeted improved YFP1-10 (T65G,T205S, T203Y) in plant cellDepositorInsertChloroplastic FNR transit peptide
UseBinaryTagsYFP1-10 (improved)ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only