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Plasmid#1026DepositorInsertPPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Cdc42-2G
Plasmid#68814PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)DepositorInsertCdc42 activation reporter
UseTagsHis and MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
mNFAT4-462:TALE:VP64:KRɸ
Plasmid#160662PurposeConstitutive expression of murine NFAT (aa 4-462) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.DepositorInsertmNFAT4-462:TALE:VP64:KRɸ
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriExRhoA2G
Plasmid#40176DepositorInsertRhoA Biosensor
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5M-TACE
Plasmid#31714DepositorInsertTACE (ADAM17 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TRBP
Plasmid#50351Purposebacterial expression of TRBPDepositorInsertTRBP (TARBP2 Human)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
hNFAT2-409:TALE:VP64:KRɸ
Plasmid#160661PurposeConstitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.DepositorInserthNFAT2-409:TALE:VP64:KRɸ
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
NbV5-HALOTAG
Plasmid#201482PurposeIndirect detection of V5-tagged protein using the HaloTag protein labeling technology.DepositorInsertNbV5-HALOTAG
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
UseTagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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PERK 2: PERK.K618A.9E10.pCDNA
Plasmid#21815DepositorInsertPERK (K618A) (Eif2ak3 Mouse)
UseTagsMyc (9E10)ExpressionMammalianMutationkinase dead K618A mutantPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAD/CMV/V5-DEST-Cdc42-2G
Plasmid#68812PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)DepositorInsertCdc42 activation reporter
UseAdenoviralTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg61-PDGFR_EF1a_eGFP
Plasmid#186310PurposePlasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg61-PDGFR
eGFP
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag3B-CCre
Plasmid#106369PurposeC-terminal part of the split-Cre SystemDepositorInsertCCre
UseCre/LoxTagsc-mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PACT
Plasmid#50352Purposebacterial expression of PACTDepositorInsertPACT (PRKRA Human)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z17-PDGFR_EF1a_mCherry
Plasmid#186303PurposePlasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ17-PDGFR
mCherry
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
PERK 3: PERK.dC.9E10.pCDNA
Plasmid#21816DepositorInsertPERK (C-term deleted) (Eif2ak3 Mouse)
UseTagsMyc (9E10)ExpressionMammalianMutationamino-acids 582-1081 deletedPromoterAvailable sinceJuly 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEFmEGFP-mDia2
Plasmid#25407DepositorInsertmDia2 (Diaph3 Mouse)
UseTagsEGFP, His, and MycExpressionMammalianMutationEncodes mDia2PromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-human-Caspase-1
Plasmid#183359PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1 (CASP1 Human)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ3M-Mst1 K59R
Plasmid#12204DepositorUseTagsMycExpressionMammalianMutationCatalytically inactive: K59RPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only