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pLVX-EGFP-IRES-Neo

Plasmid

#128660

Purpose

mammalian expression of EGFP with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

EGFP

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP

Plasmid

#194686

Purpose

CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-human Dynamin 1aa

Plasmid

#22163

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa (DNM1 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

D744N

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pGTag-NLS-eGFP-SV40

Plasmid

#117811

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

NLS

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-DIO-TC66T-2A-EGFP-2A-oG

Plasmid

#178429

Purpose

Expresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

Depositor

Edward Callaway

Inserts

TVA950 Glu66→Thr
Optimized G
EGFP

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

Expression

Mutation

Promoter

hSyn

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCCL/IFNB1-d2eGFP-3’UTR

Plasmid

#180232

Purpose

Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

Insert

d2eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

1 kb upstream of IFNB1 CDS

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEZYeGFP-SARS-CoV-2E GGG

Plasmid

#224582

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 with a mutated PBM in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E GGG (E SARS-CoV-2)

Use

Tags

GFP

Expression

Mammalian

Mutation

PBM (LLV) mutated to GGG

Promoter

CMV

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-eGFP

Plasmid

#104834

Purpose

eFFly luciferase for invivo Imaging, eGFP for flourescent detection

Depositor

Irmela Jeremias

Article

Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

Inserts

eFFly
eGFP

Use

Lentiviral and Luciferase; Egfp

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-mEGFP-YAP-MAML2

Plasmid

#217612

Purpose

part A of YAP-MAML2 SPARK-OFF

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

FKBP-mEGFP-YAP-MAML2 (YAP1, MAML2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-EGFP

Plasmid

#188900

Purpose

Human lentiviral vector for expression of EGFP from a SFFV promoter with an upstream ubiquitous chromatin opening element

Depositor

Jonathan Weissman, Marco Jost

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-FHA-SHLD2

Plasmid

#114121

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

RNF8 FHA domain (aa 2-160) and eGFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

Plasmid

#194687

Purpose

CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DRD2-cpHalo∆-mEGFP

Plasmid

#194679

Purpose

CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

DRD2_cpHalo∆

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-Stop-EGFP (MSE)

Plasmid

#154469

Purpose

MSCV-Stop-EGFP-pgk-Neo (retroviral construct containing the RFP-based translational stop cassette)

Depositor

Cornelius Miething

Article

Shoumariyeh et al Oncogene. 2020 Feb;39(9):1904-1913. doi:

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-FHA-SHLD2-m1

Plasmid

#114122

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

RNF8 FHA domain (aa 2-160) and eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5-mEGFP-HMGB1-delIDR

Plasmid

#194554

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del IDR" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

truncation by stop codon after Asn134

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG dCas9-KRAB-2A-EGFP

Plasmid

#92396

Purpose

CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9_KRAB_2A_EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-EGFP

Plasmid

#153163

Purpose

Mouse gamma-synuclein (mSncg) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mSncg

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-CBh-eGFP

Plasmid

#206885

Purpose

cloning backbone for sgRNA using OptScf2

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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