-
Plasmid#192004PurposeLentiviral vector to generate LYSET(TMEM251)-isoform1 I142V mutant stable expressing cell line under CMVTRE3G promoterDepositorInsertLYSET-Isoform1 I142V (LYSET Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVTRE3GAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMVTRE3G_LYSET-Isoform1_R45W-Puro
Plasmid#192003PurposeLentiviral vector to generate LYSET(TMEM251)-isoform1 R45W mutant stable expressing cell line under CMVTRE3G promoterDepositorInsertLYSET-Isoform1 R45W (LYSET Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVTRE3GAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMVTRE3G_LYSET-Isoform2-I142V-Flag
Plasmid#192006PurposeLentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 I142V mutant stable expressing cell line under CMVTRE3G promoterDepositorInsertLYSET-Isoform2-I142V-Flag (LYSET Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVTRE3GAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMVTRE3G_LYSET-Isoform2-R45W-Flag
Plasmid#192005PurposeLentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 R45W mutant stable expressing cell line under CMVTRE3G promoterDepositorInsertLYSET-Isoform2-R45W (LYSET Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVTRE3GAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human/mouse ELL2
Plasmid#127254PurposeExpresses a human/mouse chimera that increases ELL2 expression in mammalian cellsDepositorUseOtherTagsHis6XExpressionMammalianMutationN terminal amino acids (1-287) are human, C termi…PromoterpEF1aAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-E112A
Plasmid#110215PurposeExpression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsFlagExpressionMammalianMutationE112A, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394
Plasmid#128386PurposeExpresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruptionDepositorUseLentiviral, Luciferase, and Synthetic BiologyTagsC-terminal luc2 fragment and N-terminal luc2 frag…ExpressionMammalianMutationN terminal carbohydrate recognition domain fused …PromoterEF1A and IRESAvailable sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS1246
Plasmid#29127PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle21 (CARTPT Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1245
Plasmid#29126PurposeExpression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.DepositorInsertPle20 (CARTPT Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1243
Plasmid#29125PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle18 (CARTPT Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1492
Plasmid#29240PurposeExpression of the reporter gene was not detected in the brain and eye using LacZ.DepositorInsertPle21 (CARTPT Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSExpressionMutationPromoterAvailable sinceOct. 20, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)
Plasmid#169076PurposeInsect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepIIDepositorInsertsUseTagsGGlinker, TEV cleavage site, StrepII and HIS10 Ta…ExpressionBacterial and InsectMutationPromoterPolyhedrin promoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)
Plasmid#169077PurposeInsect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepIIDepositorInsertsUseTagsGFP tag, GGlinker, TEV cleavage site. StrepII and…ExpressionBacterial and InsectMutationPromoterPolyhedrin promoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Hu_PTTG1(WT)
Plasmid#136342PurposeMouse PTTG1 cDNA in Gateway pENTRDepositorInsertPTTG1
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Hu_CCNB2(WT)
Plasmid#136341PurposeMouse CCNB2 cDNA in Gateway pENTRDepositorInsertCCCNB2
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP1999
Plasmid#159308PurposeYeast tagging vector to create gene fusion with yeast codon optimized mCardinal under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_TetOn_Hu_PTTG1(WT)
Plasmid#136346PurposeLentiviral inducible expression (TetOn) of mouse PTTG1DepositorInsertPTTG1
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Hu_PRC1(WT)
Plasmid#136349PurposeHuman PCR1 cDNA in Gateway pENTRDepositorInsertPRC1 (PRC1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
ER-M-TAG (pGS555)
Plasmid#160040PurposepLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2DepositorInsertMMGT1-mScarlet-3xHA
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHJL4
Plasmid#199379PurposeEncodes dual cistronic cytosolic jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertjGCaMP7f::SL2::mScarlet-I
UseTagsExpressionBacterialMutationno mutationsPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only