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Showing: 3201 - 3220 of 11749 results
  1. Human/mouse ELL2

    Plasmid
    #127254
    Purpose
    Expresses a human/mouse chimera that increases ELL2 expression in mammalian cells
    Depositor
    Insert
    N-terminal human/C-terminal mouse ELL2 (ELL2, Ell2 Human, Mouse)
    Use
    Other
    Tags
    His6X
    Expression
    Mammalian
    Mutation
    N terminal amino acids (1-287) are human, C termi…
    Promoter
    pEF1a
    Available since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Insert
    SPRTN (SPRTN Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid
    #128386
    Purpose
    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption
    Depositor
    Inserts
    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Human, Synthetic)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    C-terminal luc2 fragment and N-terminal luc2 frag…
    Expression
    Mammalian
    Mutation
    N terminal carbohydrate recognition domain fused …
    Promoter
    EF1A and IRES
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)

    Plasmid
    #169076
    Purpose
    Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII
    Depositor
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    Use
    Tags
    GGlinker, TEV cleavage site, StrepII and HIS10 Ta…
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Polyhedrin promoter
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

    Plasmid
    #169077
    Purpose
    Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII
    Depositor
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    Use
    Tags
    GFP tag, GGlinker, TEV cleavage site. StrepII and…
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Polyhedrin promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pENTR_Hu_PTTG1(WT)

    Plasmid
    #136342
    Purpose
    Mouse PTTG1 cDNA in Gateway pENTR
    Insert
    PTTG1
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pENTR_Hu_CCNB2(WT)

    Plasmid
    #136341
    Purpose
    Mouse CCNB2 cDNA in Gateway pENTR
    Insert
    CCCNB2
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. FRP1999

    Plasmid
    #159308
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mCardinal under KanMX selection
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pLENTI_TetOn_Hu_PTTG1(WT)

    Plasmid
    #136346
    Purpose
    Lentiviral inducible expression (TetOn) of mouse PTTG1
    Insert
    PTTG1
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pENTR_Hu_PRC1(WT)

    Plasmid
    #136349
    Purpose
    Human PCR1 cDNA in Gateway pENTR
    Insert
    PRC1 (PRC1 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. ER-M-TAG (pGS555)

    Plasmid
    #160040
    Purpose
    pLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2
    Depositor
    Insert
    MMGT1-mScarlet-3xHA
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pHJL4

    Plasmid
    #199379
    Purpose
    Encodes dual cistronic cytosolic jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Insert
    jGCaMP7f::SL2::mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 3201 - 3220 of 11749 results