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MEK1C222
Plasmid#164638PurposeBacterial expression plasmid for His6-MEK1C222DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
UseTagsHis6-tagExpressionBacterialMutationG(-19)F/S222C/C277S/C376SPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 deltaPH
Plasmid#124922PurposeFor expression of Pleckstrin Homology domain deletion mutant of SIN1-GFP (delta376-486)DepositorInsertMAPKAP1 (MAPKAP1 Human)
UseTagsTagGFP2ExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
MEK1C218
Plasmid#164639PurposeBacterial expression plasmid for His6-MEK1C218DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
UseTagsHis6-tagExpressionBacterialMutationG(-19)F/S218C/C277S/C376SPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
MEK1C218C222
Plasmid#164640PurposeBacterial expression plasmid for His6-MEK1C218C222DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
UseTagsHis6-tagExpressionBacterialMutationG(-19)F/S218C/S222C/C277S/C376SPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Lrrc40-trunc-IRES-BFP
Plasmid#117268PurposeRetroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFPDepositorInsertLrrc40 (Lrrc40 Mouse)
UseRetroviralTagsExpressionMammalianMutationThe insert is truncated - deletion of bp 334-407 …PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
HDM-SARS2-Spike-delta21
Plasmid#155130PurposeEncodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotypingDepositorInsertSARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
UseLentiviralTagsExpressionMammalianMutationCodon optimized to H. sapiens using IDT codon opt…PromoterCMVAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/XBB.1.5-RBD
Plasmid#213038PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variantDepositorInsertpCAGGS/XBB.1.5-RBD (S )
UseTags6 His TagExpressionMammalianMutationG339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF1-Hs Cdk1/cyclin B1
Plasmid#177330PurposeCo-expression of the human active Cdk1/cyclin B1 kinase complexDepositorUseTags2 x Strep-tag and 8 x HisExpressionInsectMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Omicron.BA.5-RBD
Plasmid#213478PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variantDepositorInsertpCAGGS/Omicron.BA.5 RBD (S )
UseTags6 His TagExpressionMammalianMutationG339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/XBB.1.16-RBD
Plasmid#213070PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variantDepositorInsertpCAGGS/XBB.1.16-RBD (S )
UseTags6 His TagExpressionMammalianMutationG339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/EG5-RBD
Plasmid#213410PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variantDepositorInsertpCAGGS/EG5-RBD (S )
UseTags6 His TagExpressionMammalianMutationG339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-BA.2-AVI
Plasmid#184533PurposeExpresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-BA.2 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationG339D, S371F, S373P, S375F, T376A, D405N, R408S, …PromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BECN1_WT
Plasmid#82100PurposeGateway Donor vector containing BECN1 , part of the Target Accelerator Plasmid Collection.DepositorInsertBECN1 (BECN1 Human)
UseGateway entry vectorTagsExpressionMutationA103VPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pαH-S-GSAS-2P-Omicron-BA.5
Plasmid#213828PurposeMammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variantDepositorInsertpαH-S-GSAS/Omicron-2P-BA5 (S )
UseTagsHRV 3C cleavage site (before tags) 8X His tag 2X…ExpressionMammalianMutationG339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-BJ.1-AVI
Plasmid#196617PurposeExpresses SARS-CoV-2 RBD protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-RBD-BJ.1
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationG339H, R346T, L368I, S371F, S373P, S375F, T376A, …PromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD G-Loop1
Plasmid#188128PurposeExpresses C-terminal flag-tagged human CAD G-Loop1 in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Δ1-60MEK1C222
Plasmid#164641PurposeBacterial expression plasmid for His6-ΔMEK1C222DepositorInsertN-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
UseTagsHis6-tagExpressionBacterialMutationS222C/C277S/C376S; residues 1-60 at N-terminus we…PromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Δ1-60MEK1C218
Plasmid#164643PurposeBacterial expression plasmid for His6-ΔMEK1C218DepositorInsertN-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
UseTagsHis6-tagExpressionBacterialMutationS218C/C277S/C376S; residues 1-60 at N-terminus we…PromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only
