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Showing: 6401 - 6440 of 156554 results

  1. pFRT-TODestGFP_RNAseH1

    Plasmid
    #65784
    Purpose
    expresses GFP-tagged RNAseH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Zebrafish, Human)
    Use
    Tags
    GFP and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65784-plasmid-map-sequence-id-111845

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  5. pSL1-GFP

    Plasmid
    #180420
    Purpose
    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.
    Depositor
    Nancy Moran
    Article
    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119
    Insert
    GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    180420-plasmid-map-sequence-id-357217

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  6. R777-E015 Hs.BRAF

    Plasmid
    #70299
    Purpose
    Gateway ORF clone of human BRAF [NM_004333.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    BRAF (BRAF Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70299-plasmid-map-sequence-id-125748

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  9. NTSR1-DuET

    Plasmid
    #213356
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    NTSR1 (NTSR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213356-plasmid-map-sequence-id-430917

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  10. pTrig-CopA

    Plasmid
    #104578
    Purpose
    Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under CopA promoter for copy increase.
    Depositor
    Harris Wang
    Article
    Sheth et al Science. 2017 Dec 15;358(6369):1457-1461
    Insert
    PCopA-RiboJ-B0034-repL
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104578-plasmid-map-sequence-id-202345

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  14. pmCellFree_KA1_SARS-COV-2_S

    Plasmid
    #169402
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus S with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _S (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    169402-plasmid-map-sequence-id-336167

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  15. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-260007

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  17. pJRA109

    Plasmid
    #209342
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mTagBFP2 fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209342-plasmid-map-sequence-id-420463

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  19. 4xmts-mNeonGreen

    Plasmid
    #98876
    Purpose
    In vivo visualization of mitochondria (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    4xmts
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    98876-plasmid-map-sequence-id-194881

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  29. pAd5-B6/7

    Plasmid
    #175747
    Purpose
    A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6 and pAd5-B7.
    Depositor
    Fred Bunz
    Article
    Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do
    Insert
    Adenovirus 5 genomic region 25043-35938
    Use
    Adenoviral and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    175747-plasmid-map-sequence-id-345606

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  31. TFORF3415

    Plasmid
    #144891
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NKRF (NKRF Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144891-plasmid-map-sequence-id-404481

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  32. pAAV MP-135 mRFP

    Plasmid
    #206927
    Purpose
    The micro promoter MP-135 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (135 bp) allows delivery of large genes in AAV.
    Depositor
    Markus Grompe
    Article
    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2
    Insert
    mRFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MP-135
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206927-plasmid-map-sequence-id-407771

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  35. gCBEv2

    Plasmid
    #220617
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220617-plasmid-map-sequence-id-436955

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  36. PS-CFP2-TOMM20-N-10

    Plasmid
    #57250
    Purpose
    Localization: Outer Mitochondrial Membrane, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Use
    Tags
    PS-CFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 13, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  37. mEmerald-IFT88-N-18

    Plasmid
    #54125
    Purpose
    Localization: Cilium, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    IFT88 (Ift88 Mouse)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    N241D in IFT88
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54125-plasmid-map-sequence-id-340533

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Showing: 6401 - 6440 of 156554 results