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Plasmid#122205PurposeBipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.DepositorInsertdCas9-KRAB-MeCP2
UseLentiviralTagsKRAB-MeCP2ExpressionMammalianMutationCas9 (D10A, H840A)PromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-flpE-puro
Plasmid#20733DepositorInsertFlpE recombinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHIPH4
Plasmid#117685PurposeHansenula polymorpha expression plasmidDepositorInsertAlcohol Oxidase promoter
UseTagsExpressionYeastMutationPromoterAlcohol OxidaseAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330 p53
Plasmid#59910PurposepX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoterDepositorInsertsgRNA targeting p53 (Trp53 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCdOpt-BMX
Plasmid#203929PurposeThis plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiaeDepositorInsertBleMX
UseTagsExpressionYeastMutationBleMX was codon optimized for Candida albicans or…PromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X
Plasmid#192148PurposeT cell CRISPR AAV vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Shp2 biosensor
Plasmid#134346PurposeFRET biosensor. To visualize Shp2 activity by FRETDepositorInsertShp2 Biosensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP×3-N1
Plasmid#86776PurposeA mammalian expression vector with triple EGFP at C-terminus.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY004 (pcDNA3.1-hFnCpf1)
Plasmid#69976PurposeExpresses humanized FnCpf1DepositorInserthFnCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)-mCherry
Plasmid#50474PurposeGq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH145
Plasmid#68380PurposeIMCE attB donor copy control fosmidDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG
Plasmid#176395PurposeExpresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.DepositorInsertGAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a PhoCl-TsCC(B)::KANMX
Plasmid#185906PurposePlasmid for cloning PhoCl fused to TsCC(B) into yeast.DepositorInsertPhoCl-TsCC(B)
UseTagsPhoCl fluorophore with C-terminal TsCC(B)ExpressionYeastMutationPromoterSP6Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSncg-Cre
Plasmid#153207PurposeMouse gamma-synuclein (mSncg) promoter-mediates Cre expression in retinal ganglion cellsDepositorInsertCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromotermSncgAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Atg12
Plasmid#24923DepositorInsertAtg12 (ATG12 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XMyc-EGFP-PEX26
Plasmid#139059PurposeMyc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XMyc-EGFP-PEX26
UseLentiviralTagsMycExpressionMutationPromoterUBCAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
gGBEv6.3-SpCas9
Plasmid#202629Purposevector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertnSpCas9(D10A)-MPGv6.3
UseTagsExpressionMammalianMutationMPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…PromoterhU6, EF1a, CBhAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 3xFLAG TPP1 88-544
Plasmid#53548Purposemammalian expression of TPP1 full lengthDepositorInsertTPP1 (ACD Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
eGFP-luciferase-C1
Plasmid#163523PurposeExpresses both eGFP and firefly luciferase in mammalian cellsDepositorInsertFirefly luciferase
UseLuciferaseTagseGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-Mango II x 24
Plasmid#127587PurposeExpresses the mCherry-Mango II x 24 array from a TetON responsive promoterDepositorInsertmCherry-Mango II x24
UseTagsmCherryExpressionMammalianMutationPromoterpTRE Tet ONAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_USP10_C424A
Plasmid#127103DepositorInsertUSP10 (USP10 Human)
UseTagsExpressionMammalianMutationC424APromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR CD19-PI3K CAR
Plasmid#113018Purposefor lentivirus production of CD19-PI3K CARDepositorInsertCD19 CAR-P intracellular PI3k recruiting domain from CD19 (CD19 Human, Synthetic)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580APromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK067
Plasmid#65174PurposeEncodes ConR1 as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConR1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam E2F4
Plasmid#37966DepositorInsertE2F4 (E2F4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-CEBPA
Plasmid#219393PurposeExpress CEBPA in mammalian cellsDepositorInsertCEBPA (CEBPA Human)
UseTagsN terminal Myc tagExpressionMammalianMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-3xFLAG
Plasmid#207684PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
TERT - WT
Plasmid#213827PurposeExpresses human telomerase reverse transcriptase, the catalytic subunit of telomeraseDepositorInserthuman TERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTet-responsive promoter PTight, consisting of sev…Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-PIGA (pLP140)
Plasmid#168461PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
UseTags6xHis, E-tag, and PelB signal peptideExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only