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Plasmid#14846DepositorInsertPHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-A1T6. TATA box mutated from TATATAAG to AATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pGLx2-set1deltaRRM
Plasmid#53257PurposeGAL driven LexA-tagged SET1-deltaRRM (RRM domain deleted)DepositorInsertSET1
UseTagsLexAExpressionYeastMutationRRM domain deleted (aa 230-355)PromoterGAL1/10Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME-basGal80
Plasmid#51621Purposevertebrate expression of Gal80 preceded by a basal promoterDepositorInsertGal80 (GAL80 Budding Yeast)
UseVertebrate expressionTagsExpressionMutationPromoterbasal promoterAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLJD vector
Plasmid#34595DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL2 enhancer- F4 LUC
Plasmid#22434DepositorInsertF4 (LOC110973015 Human)
UseLuciferaseTagsluciferase reporter geneExpressionMammalianMutationPromoterAvailable sinceMay 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL2 enhancer- F5 LUC
Plasmid#22436DepositorInsertF5 (LOC110973015 Human)
UseLuciferaseTagsluciferase reporter geneExpressionMammalianMutationPromoterAvailable sinceMay 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 PHO5(UASm1)pr VYFP (EB#1549)
Plasmid#14841DepositorInsertPHO5 promoter UASm1 (PHO5 Budding Yeast)
UseTagsVYFPExpressionYeastMutationUASm1 (AAGCTT was substituted for CACGTT). Unindu…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)
Plasmid#14844DepositorInsertPHO5 promoter TATA-C2 (PHO5 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-C2. TATA box mutated from TATATAAG to TCTA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSEVA47_dCas9-GFP_YES_i2
Plasmid#231419PurposeThis YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.DepositorInsertsdCas9
GFP
sgRNA-Y
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-Pcym
Plasmid#225280PurposeMini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPcym cumate (p-isopropylbenzoate)-inducible promo…Available sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-PcL
Plasmid#225281PurposeMini-Tn7 pGP-Tn7-Cm vector with constitutive PcL promoter for the constitutive expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterConstitutive PcL promoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-PrpsM
Plasmid#225282PurposeMini-Tn7 pGP-Tn7-Cm vector with constitutive PrpsM promoter for the constitutive expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterConstitutive PrpsM promoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSJ105
Plasmid#208859PurposeExpresses SoxS activation domain and gRNA targeting 105 region within the synthetic promoter in bacterial cellDepositorInsertsgRNA 105
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterCRISPR/dCas9-responsive synthetic promoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM315 pACYC-His6-Rpn10[ΔUIM]
Plasmid#226348PurposeExpression of yeast Rpn10 with the UIM deletedDepositorInsertrpn10 (RPN10 Budding Yeast)
UseTagsHisExpressionBacterialMutationdelta UIMPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-DNM1L -GFP
Plasmid#221845PurposeTol2 transposon expressing sgRNA targeting chick DNM1L from chick U6.3 promoter expresses GFP reporter from GAGC promoterDepositorInsertsEGFP
DNM1L sgRNA-gTGTTTTCCGACCATCCTCTG
UseCRISPR; Tol2 transposon optimised for chick expre…TagsExpressionMammalianMutationPromoterCAGC and U6.3 chickAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-MFN1-GFP
Plasmid#221846PurposeTol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promoteDepositorInsertsEGFP
MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT
UseCRISPR; Tol2 transposon optimised for chick expre…TagsExpressionMammalianMutationPromoterCAGC and U6.3 chickAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM024_P2_pMPA1
Plasmid#216449PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter mpa1
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM022_P2_pRPS1002
Plasmid#216447PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter rps1002
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM020_P2_pTRS1
Plasmid#216445PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter trs1
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only