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Plasmid#71350Purposecsd6, construct:1-330, pET28b(+) N-tagDepositorInsertHP0518 (HP0518 )
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF3-V5-His6
Plasmid#67412PurposeExpression of hArf3 with C-term V5 and His6 tag in E. coliDepositorInsertARF3 (ARF3 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF4-V5-His6
Plasmid#67413PurposeExpression of hArf4 with C-term V5 and His6 tag in E. coliDepositorInsertARF4 (ARF4 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF5-V5-His6
Plasmid#67414PurposeExpression of hArf5 with C-term V5 and His6 tag in E. coliDepositorInsertARF5 (ARF5 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL1-V5-His6
Plasmid#67416PurposeExpression of hArl1 with C-term V5 and His6 tag in E. coliDepositorInsertARL1 (ARL1 Human)
UseTagsHis and V5ExpressionBacterialMutationbp 231 C to T; silent mutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL2-V5-His6
Plasmid#67417PurposeExpression of hArl2 with C-term V5 and His6 tag in E. coliDepositorInsertARL2 (ARL2 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL5A-V5-His6
Plasmid#67420PurposeExpression of hArl5 with C-term V5 and His6 tag in E. coliDepositorInsertARL5A (ARL5A Human)
UseTagsHis and V5ExpressionBacterialMutationbp 70 C to T; silent mutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL8A-V5-His6
Plasmid#67424PurposeExpression of hArl8 with C-term V5 and His6 tag in E. coliDepositorInsertARL8A (ARL8A Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL4D-V5-His6
Plasmid#67426PurposeExpression of hArl4D with C-term V5 and His6 tag in E. coliDepositorInsertARL4D (ARL4D Human)
UseTagsHis and V5ExpressionBacterialMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL14-V5-His6
Plasmid#67427PurposeExpression of hArl14 with C-term V5 and His6 tag in E. coliDepositorInsertARL14 (ARL14 Human)
UseTagsHis and V5ExpressionBacterialMutationbp 172 C to T; aa L58FPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL11-V5-His6
Plasmid#67428PurposeExpression of hArl11 with C-term V5 and His6 tag in E. coliDepositorInsertARL11 (ARL11 Human)
UseTagsHis and V5ExpressionBacterialMutationbp 442 T to C; aa C148RPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARFRP1-V5-His6
Plasmid#67429PurposeExpression of hArfrp with C-term V5 and His6 tag in E. coliDepositorInsertARFRP1 (ARFRP1 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-SBL1(160S,HisTagDel)
Plasmid#66880PurposeExpresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removedDepositorInsertsoybean lipoxygenase-1 (S160S)
UseTagsnoneExpressionBacterialMutationnonePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-SBL1(160E,HisTagDel)
Plasmid#66879PurposeExpresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removedDepositorInsertsoybean lipoxygenase-1 (S160E)
UseTagsExpressionBacterialMutationS160EPromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S,NoCys)
Plasmid#66877PurposeExpresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1
UseTagsHistagExpressionBacterialMutationfour (all) cysteines mutated to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E,NoCys)
Plasmid#66875PurposeExpresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1 (S160E) with all Cys to Ser mutations
UseTagsHistagExpressionBacterialMutationS160E; changed 4 (all) cysteines to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(T214V)
Plasmid#66137PurposeExpresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationT214VPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 G108C/P186C
Plasmid#66174PurposeExpresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationG108C, P186CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Guinea Pig 1-88)
Plasmid#62059PurposeExpression of Guinea Pig p53 (residues 1-88) in e. coliDepositorInsertp53
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-88PromoterT7Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-T7-Ntx-K49
Plasmid#58715PurposeExpresses in Escherichia coli T7-Notexin with the amino acid substitution D49KDepositorInsertNotexin
UseTagsT7 peptide tagExpressionBacterialMutationChanged Aspartic Acid 49 to LysinePromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only