Addgene

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pGEX Smad3 NL

Plasmid

#12632

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 NL (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

N-L segment of Smad3 (aa 1-211)

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pDEST51-LRRK2-R1441H

Plasmid

#25083

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arg 1441 mutated to His

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pCIneo-lambdaN-HA-HsTNRC6A_C

Plasmid

#146086

Purpose

Mammalian Expression of HsTNRC6A

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A (TNRC6A Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-FAK

Plasmid

#74478

Purpose

This construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.

Depositor

J Thomas Parsons

Article

Schaller et al Proc Natl Acad Sci U S A. 1992 Jun 1;89(

Insert

FAK (PTK2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZ414-F31

Plasmid

#23067

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed lysine 14 to Glutamine K14Q

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
RV-Syn-Dio-GFP

Plasmid

#87673

Purpose

retroviral vector encoding cre dependent expression of GFP

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

eGFP

Use

Retroviral

Tags

Expression

Mutation

Promoter

Synapsin

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAK10-His-Myc-Kal9

Plasmid

#25441

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal9

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pAF1-VP64-SeqFosA

Plasmid

#51430

Purpose

Artificial transcription factor with VP64 targeting c-Fos (TALE A)

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pGL2 enhancer- F1 LUC

Plasmid

#22426

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

eNOS promotor F1 (NOS3 Human)

Use

Luciferase

Tags

Luciferase reporter gene

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only
Kohinoor-Golgi/pcDNA3

Plasmid

#67779

Purpose

Golgi-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

GT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmTral

Plasmid

#21722

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Trailer hitch (tral Fly)

Use

Drosophila expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW-EGFP-4xlet-7 (pAW-AS27)

Plasmid

#40834

Purpose

miRNA sensor used in Drosophila cell lines

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

Four target sites perfectly complementary to let-7

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
EZ-L498

Plasmid

#131771

Purpose

PPGK1_FUSN_Citrine _PixE_TCYC1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PPGK1_FUSN_Citrine _PixE_TCYC1

Use

Integration into his3 locus

Tags

Expression

Yeast

Mutation

Promoter

PGK

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEAK10-His-Myc-Kal5

Plasmid

#25440

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal5

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pUC_h7SK Mma PylT (UUA)

Plasmid

#154783

Purpose

plasmid with single copy of Mma PylT (UUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Use

Tags

Expression

Mammalian

Mutation

U25C and UUA anticodon

Promoter

h7SK

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-HBH

Plasmid

#26885

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag N-terminal

Use

Pcr tagging yeast

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pNVLTv2-pvdIJD-Prha-base

Plasmid

#169244

Purpose

Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

rhaRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhaS

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEE386

Plasmid

#149284

Purpose

T-DNA encoding TRV2 with gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEE388

Plasmid

#149286

Purpose

T-DNA encoding TRV2 with mFT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_mFT:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
Kohinoor-Zyxin/pcDNA3

Plasmid

#67777

Purpose

Zyxin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Zyxin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only