Addgene

Showing: 3181 - 3200 of 3890 results

Results per page:

Sort results by:

pCMV-PE7-P2A-BSD

Plasmid

#224122

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-BSD

Use

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

Plasmid

#67980

Purpose

Cas9 activity reporter with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PE7_AAVS1_knockin_HDR_donor

Plasmid

#223001

Purpose

HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-APP-mGFP

Plasmid

#196696

Purpose

Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Use

Tags

HA and mGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1-eBFP2

Plasmid

#102640

Purpose

In vitro expression of eBFP2 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

eBFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCG_SARS-CoV1 N C-V5-tag

Plasmid

#179968

Purpose

Mammalian expression vector for SARS-CoV-1 N, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 N (N Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCG_SARS-CoV1 C-V5-tag ORF3a

Plasmid

#179969

Purpose

Mammalian expression vector for SARS-CoV-1 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 ORF3a (ORF3a Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCG_batCoV RaTG13 ORF3a C-V5-tag

Plasmid

#179988

Purpose

Mammalian expression vector for RaTG13 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 ORF3a

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1-mTFP1

Plasmid

#102635

Purpose

In vitro expression of mTFP1 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mTFP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GAPDH

Plasmid

#155622

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

GAPDH (GAPDH Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-BACE1-mBFP

Plasmid

#196697

Purpose

Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Beta-secretase 1 (BACE1 Human)

Use

Tags

HA and mBFP

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

Plasmid

#207507

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-hIKAROS-IRES-GFP

Plasmid

#74046

Purpose

retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Ikaros (IKZF1) (IKZF1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDY0216 PASTEv1 PE2-P2A-Bxb1

Plasmid

#179104

Purpose

PASTEv1 PE2-P2A-Bxb1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-PE2-P2A-Bxb1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCG_SARS-CoV2 ORF3a C-V5-tag

Plasmid

#179980

Purpose

Mammalian expression vector for SARS-CoV-2 ORF3a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-2 ORF3a (ORF3a Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCG_SARS-CoV1 NSP3 C-V5-tag

Plasmid

#179962

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp3, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 Nsp3 (ORF1ab Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1-mRFP1

Plasmid

#102630

Purpose

In vitro expression of mRFP1 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mRFP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSAM2_mCherry_Gata6

Plasmid

#72694

Purpose

Doxycyline regulated expression of Gata6 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 6 (Gata6 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDY0207 ACTB atgRNA with v1 scaffold

Plasmid

#179107

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Promoter-hPE(+)-Luc

Plasmid

#172003

Purpose

Luciferase Reporter for Human PTEN Enhancer PE - Forward Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Human PE sequence (hPE)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

SV40 Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge