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Plasmid#220241PurposeExpression of an EZH2 catalytic site mutant in insect cells.DepositorInsertdEZH2 (EZH2 Human)
UseTags6x His, MBPExpressionInsectMutationY731FPromoterPolyhedrinAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt2
Plasmid#220240PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
UseTags6x His, MBPExpressionInsectMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterPolyhedrinAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt1
Plasmid#220239PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 1 (EZH2 Human)
UseTags6x His, MBPExpressionInsectMutationR27A, R31A, F165APromoterPolyhedrinAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_NEUROD1loop
Plasmid#215032PurposeMammalian expression of human TWIST1 (with NEUROD1 loop)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop (aa134-141, IIPTLPSD) replaced w…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_NEUROG2loop
Plasmid#215033PurposeMammalian expression of human TWIST1 (with NEUROG2 loop)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop (aa137-141, TLPSD) replaced with…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_HAND2loop
Plasmid#215034PurposeMammalian expression of human TWIST1 (with HAND2 loop)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop (aa137-141, TLPSD) replaced with…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_TAL1loop
Plasmid#215035PurposeMammalian expression of human TWIST1 (with TAL1 loop)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationTWIST1 bHLH loop (aa137-141, TLPSD) replaced with…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_NEUROD1M
Plasmid#215045PurposeMammalian expression of human TWIST1 (with fragment of loop from NEUROD1)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationMiddle portion of TWIST1 bHLH loop (aa139, P) rep…PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EFNB2 (D62Q)
Plasmid#200976PurposeMammalian expression plasmid for myc-tagged EFNB2 (D62Q specificity mutant)DepositorInsertEFNB2 (EFNB2 Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationD62Q (increases specificity towards henipaviral G…PromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ERBB4-V5/HIS
Plasmid#201105Purposeexpression of human ERBB4 receptor tyrosine kinase in mammalian cellsDepositorInserthuman ERBB4 receptor tyrosine kinase, full length, wildtype (ERBB4 Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
CIRC
Plasmid#186343PurposeContains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)DepositorInsertVEGFA (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-cDAXX-0SB
Plasmid#169730PurposeBacterial expression of N-terminally 6His tagged cDAXX-0SBDepositorInsertcDAXX-0SB (DAXX Human)
UseTags6 HisExpressionBacterialMutationComprises residues 495-741 and has the major SB m…PromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDEST53-LRRK2-3XKD
Plasmid#25049DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutation"Kinase Dead" Lysine 1906 mutated to A…PromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)
Plasmid#69138PurposeFor the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivoDepositorInsertCAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
UseTagsnoneExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EGFR-V5/HIS
Plasmid#201102Purposeexpression of human EGFR receptor tyrosine kinase in mammalian cellsDepositorInserthuman EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VEGFR3-V5/HIS
Plasmid#205618PurposeExpression of human VEGFR3 receptor tyrosine kinase in mammalian cellsDepositorInsertVEGFR3 receptor tyrosine kinase (FLT4 Human)
UseTagsV5-HisExpressionMammalianMutationT28M, S455R- Please see depositor commentPromoterCMVAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FGFR2c-V5/HIS
Plasmid#201107Purposeexpression of human FGFR2 receptor tyrosine kinase in mammalian cellsDepositorInserthuman FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-WT(732)-IgG1
Plasmid#154104PurposeMammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-WT(732)-8h
Plasmid#154101PurposeMammalian expression plasmid for dimeric soluble ACE2 (8his tag)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only