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pAAVS1-Neo-TNNT2-Puro

Plasmid

#214016

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Zeo

Plasmid

#214017

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hORAI1sg

Plasmid

#174326

Purpose

gRNA targeting hORAI1 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.3a

Plasmid

#111055

Purpose

Expresses dLight1.3a in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.3a

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER-hHSF2 shRNA

Plasmid

#118346

Purpose

This plasmid expresses shRNA against hHSF2.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF2 shRNA (HSF2 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHDR-eGFP (pXK-1647)

Plasmid

#208824

Purpose

The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR

Depositor

Kun Xu

Article

Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-mqgDUX4mqg*

Plasmid

#21177

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Apr 9. ():.

Insert

DUX4 (DUX4 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Point mutation at nucleotide 6163 (numbering acco…

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pPBpuro-mNG-eDHFR(69K6)-Tiam1

Plasmid

#172102

Purpose

Mammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Tiam1(DH-PH) (TIAM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8-112–858_Flag2-Blast

Plasmid

#175039

Purpose

Mammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein from amino acid positi…

Promoter

CAG

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8-1–729_Flag2-Blast

Plasmid

#175040

Purpose

Mammalian expression of mMPP8 (M1 to E729) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein from amino acid positi…

Promoter

CAG

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8-∆ARD_Flag2-Blast

Plasmid

#175041

Purpose

Mammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein with deletion of the a…

Promoter

CAG

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCA-G-intron(Neo)-T(FLLFLNL)

Plasmid

#40020

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdT-3Myc
beta-globin intron
Neo

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pBT259_(pRosa26-G-tTA2)

Plasmid

#36878

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
diphteria toxin A

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pBT346.6-H3.3-K4M-bGHpolyA

Plasmid

#128743

Purpose

Inducible expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

K4M

Promoter

CAG

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4mqg*

Plasmid

#21175

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Apr 9. ():.

Insert

DUX4 (DUX4 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Point mutation at nucleotide 6163 (numbering acco…

Promoter

Available since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only
pBT250_(pCA-G-intron(Neo)-tTA2)

Plasmid

#36876

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MSP1594_2x_NLS

Plasmid

#110625

Purpose

Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
None

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCA-G-intron(Neo)-T(LNL)

Plasmid

#36907

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdTomato-3Myc
beta-globin intron
Neo

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-polyQ74-NLS

Plasmid

#231892

Purpose

Forms nuclear HTT partial exon 1 Q74-NLS aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74-NLS (HTT Human)

Use

Tags

EGFP and SV40 NLS

Expression

Mammalian

Mutation

CAG repeat expansion

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCIG2-IRES-Lamp1-mNeon Green

Plasmid

#219441

Purpose

Expresses Lamp1 fused to mNeon Green

Depositor

Erika Holzbaur

Article

Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

Insert

LAMP1 (Lamp1 Rat)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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