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Danio alpha A-crystallin 1kb promoter/AcGFP
Plasmid#98096PurposeaA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha A-crystallin 1 kb fragment (-1028/-1)Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry C1 MFF
Plasmid#157760PurposeExpression of mcherry:MFF in cellsDepositorInsertMFF (MFF Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-T1977R
Plasmid#69011Purposeactivating MTOR mutationDepositorInsertMTOR-T1977R (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Threonine 1977 to ArgininePromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR55
Plasmid#106026PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR55; Opto-GPR55; C4)DepositorInsertOpto-GPR55 (GPR55 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
F5-bio-His
Plasmid#53426PurposeExpresses full-length Coagulation factor V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertF5 (F5 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB7
Plasmid#63108PurposeExpression of HSPB7 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb7 (HSPB7 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hKCNQ1
Plasmid#53048PurposeExpresses alpha subunit for hKCNQ1 channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)
UseXenopus oocyte expressionTagsExpressionMutationPromoterSP6Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-hXRCC1-mCherry-Hygro
Plasmid#176532PurposemCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassetteDepositorInsertX-Ray Repair Cross Complementing 1 (XRCC1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-H1.4
Plasmid#157799PurposeExpression of mEGFP and human linker histone H1.4 fusion proteinDepositorInsertmEGFP-H1.4 (H1-4 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1_6xHis-TEV-GABARAP-Gly
Plasmid#190930PurposePlasmid for the expression and purification of GABARAP. Internal Reference: SMC894DepositorInsertGABARAP (GABARAP Human)
UseTags6xHis-TEVExpressionBacterialMutationEnds with Glycine 116PromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124431PurposeExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafishDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseZebrafish (if expression level is too high in zeb…TagsExpressionMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PVRL2-bio-His
Plasmid#51824PurposeExpresses full-length Poliovirus receptor-related protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPVRL2 (NECTIN2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-SSeCKs T751A
Plasmid#107142Purposefull-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vectorDepositorInsertSSeCKs T751A (Akap12 Mouse)
UseRetroviralTagsExpressionMammalianMutationchanged threonine 751 to alaninePromotergagAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG-VP16–RAR-alpha
Plasmid#80434PurposeExpression of constitutively active human RAR-alpha in chick embryosDepositorInsertRetinoic acid receptor alpha (RARA Human)
UseTagsEGFP and VP16ExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR4
Plasmid#106001PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR4; Opto-GPR4; A3)DepositorInsertOpto-GPR4 (GPR4 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1_HBB_N
Plasmid#87847PurposepCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNADepositorInsertHomo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
TMED10-bio-His
Plasmid#51852PurposeExpresses full-length Transmembrane emp24 domain-containing protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED10 (TMED10 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-F1888L
Plasmid#69010Purposeactivating MTOR mutationDepositorInsertMTOR-F1888L (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Phenylalanine 1888 to LeucinePromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
TMED5-bio-His
Plasmid#53338PurposeExpresses full-length Transmembrane emp24 domain-containing protein 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED5 (TMED5 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC39A8
Plasmid#132177PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC39A8 (SLC39A8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
ACVR1B-bio-His
Plasmid#51640PurposeExpresses full-length Activin receptor type 1B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertACVR1B (ACVR1B Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC39A10
Plasmid#132275PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC39A10 (SLC39A10 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-SYN_mCyRFP2-CREB
Plasmid#137008PurposeAAV backbone, mCyRFP2-CREB donor for R-CREBDepositorInsertCREB1 (Creb1 Rat)
UseAAVTagsmCyRFP2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB6
Plasmid#63107PurposeExpression of HSPB6 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb6 (HSPB6 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA
Plasmid#192160PurposeDual AAV vector AAV-dCas9DepositorInsertDead Cas9
UseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PTGS1-bio-His
Plasmid#53414PurposeExpresses full-length Prostaglandin G/H synthase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPTGS1 (PTGS1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB4
Plasmid#63105PurposeExpression of HSPB4 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb4 (CRYAA Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR3
Plasmid#106000PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR3; Opto-GPR3; A2)DepositorInsertOpto-GPR3 (GPR3 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiDP1.0
Plasmid#171094Purposelentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PDPN-bio-His
Plasmid#52313PurposeExpresses full-length Podoplanin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPDPN (PDPN Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
ABE8.17-d
Plasmid#136299Purposeexpresses ABE8.17-d in mammalian cellsDepositorInsertABE8.20-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-221-PGK-GFP-Blast
Plasmid#31519DepositorInsertmiR-221 (Mir221 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CRY2-mCherry
Plasmid#140571PurposeFor use in light-induced protein inactivation through the interaction with CIB1DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMV IE94Available sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
F2-bio-His
Plasmid#52179PurposeExpresses full-length Prothrombin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertF2 (F2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNT.2#2/Cre
Plasmid#173661PurposeExpresses a NT.2-targeting gRNA and Cre-recombinaseDepositorInsertNon-targeting gRNA
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-LmoCas6
Plasmid#126485PurposeEncodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas6
UseCRISPRTagsFlag, NLSExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide-Puro/CD73-gRNA4
Plasmid#132588PurposeLentiviral gRNA vector targeting human CD73DepositorInsertCD73 (NT5E Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
