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psgRNA

Plasmid

#114005

Purpose

express sgRNA. ColE1, Kan

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

BBa_J23119

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Ef1a_Large T-antigen_Ires_Puro

Plasmid

#18922

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

Large T Antigen (SV40gp6 Simian virus 40)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-Q74

Plasmid

#40262

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q74 (HTT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-flag-Lag16-iCAM-1

Plasmid

#205195

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag16 anti-GFP nanobody

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MAT2A

Plasmid

#100521

Purpose

Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

MAT2A (MAT2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pTight TRE

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-Neo-M2rtTA

Plasmid

#60843

Purpose

AAVS1 donor vector for genomic targeting

Depositor

Rudolf Jaenisch

Article

DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

Inserts

M2rtTA
Neo

Use

Targeting donor

Tags

Expression

Mutation

Promoter

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Dio-GFP

Plasmid

#198279

Purpose

Cre reporter for mammalian cells; double-floxxed-EGFP

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Dio-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

Plasmid

#188777

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Jonathan Weissman, Marco Jost

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Expression

Mutation

Promoter

EF1a

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pEZYmyc-His

Plasmid

#18701

Purpose

pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Plasmid

#132543

Purpose

Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

cmv d0

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-2xFYVE

Plasmid

#140050

Purpose

Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

Depositor

Harald Stenmark

Article

Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Promoter

CMV

Available since

May 3, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-kdrl

Plasmid

#78687

Purpose

kdrl promoter for Gateway cloning

Depositor

Sarah Childs, Jau-Nian Chen

Article

Choi et al Dev Biol. 2007 Apr 15;304(2):735-44. Epu

Insert

kdrl promoter (kdrl Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

kdrl

Available since

June 5, 2016

Availability

Academic Institutions and Nonprofits only

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EB3-mNeonGreen

Plasmid

#98881

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

EB3

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGLO-GFP-1UAG

Plasmid

#82500

Purpose

Expresses GFP with 1 UAG codon at the amino acid position 3

Depositor

Chang Liu

Article

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Green Fluorescent Protein with 1 UAG codon

Use

Tags

Expression

Bacterial

Mutation

Added an UAG codon at the amino acid position 3

Promoter

AraBAD

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Map3k1

Plasmid

#29581

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Map3k1 (MAP3K1 Human)

Use

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Constitutively Active. Contains catalytic domain …

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
LV-hGFAP-GFP

Plasmid

#183906

Purpose

Expression of GFP under the human GFAP ABC1D promoter

Depositor

Chun-Li Zhang

Article

Niu et al Nat Cell Biol. 2013 Oct;15(10):1164-75.

Insert

GFP (GFAP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDq-P2A-mCherry

Plasmid

#204357

Purpose

AAV vector for miniDq expression under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Reports Methods, Volume 4, Issue 10

Insert

miniDq-P2A-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

The third intracellular loop (ICL3) of hM3Dq was …

Promoter

hSyn

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLGB30

Plasmid

#126620

Purpose

Suicide vector for allelic replacement in Bacteroides, tetracycline selection and rhamnose-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Tags

Expression

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING

Plasmid

#102586

Purpose

Lentivector to express STING and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSW149

Plasmid

#122248

Purpose

pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.

Depositor

Sarah Walker

Article

Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

Insert

TIF4631 (TIF4631 Budding Yeast)

Use

Tags

Intein-Chitin-binding domain

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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