Addgene

We narrowed to 758 results for: xenopus

Showing: 301 - 320 of 758 results

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pCS2+-wbp2nl

Plasmid

#172463

Purpose

expresses wild-type protein when injected into embryos

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl (wbp2nl.L Frog)

Use

Xenopus expression

Tags

none

Expression

Mutation

none

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlY55F

Plasmid

#172466

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY55F

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Tyrosine at aa55 to Phenylalanine

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlY282F

Plasmid

#172468

Purpose

expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlY282F

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Tyrosine at aa282 to Phenylalanine

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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pCS2 Bighead S-HA

Plasmid

#172485

Purpose

for synthesis of mRNA for injection into xenopus laevis embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

In vitro mrna synthesis

Tags

HA and S-HA

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-A6

Plasmid

#185506

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

FSIENIM to AAAAAAM

Promoter

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB2

Plasmid

#185512

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

IDIL replaced with AAAAAA

Promoter

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB4

Plasmid

#185513

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Expression

Mutation

IDILGE replaced with AAAAAA

Promoter

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nlF127P

Plasmid

#174507

Purpose

expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nlF127P

Use

Xenopus expression

Tags

none

Expression

Mutation

changed Phenylalanine at aa127 to Proline

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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pET3a-XlH3[ChEx]

Plasmid

#221200

Purpose

Xenopus laevis histone H3 with Nterminal tail swapped for ChEx domain of ScChd1.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3[ChEx]

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET3a-Xl-H3∆Nterm[R42]

Plasmid

#221201

Purpose

Xenopus laevis histone H3 with N-terminal tail deleted.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3∆Nterm[R42]

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT7TS-rich

Plasmid

#99050

Purpose

This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.

Depositor

Richard Bélanger

Type

Empty backbone

Use

Unspecified; Xenopus oocyte system

Tags

Expression

Mutation

Promoter

pT7

Availability

Academic Institutions and Nonprofits only

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rGSKmyc-R85-pXT7

Plasmid

#15493

Depositor

Sergei Sokol

Article

Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

Insert

GSK-3 beta R85 (Gsk3b Rat)

Use

Xenopus rna expression

Tags

myc

Expression

Mutation

R85: K85R, dominant negative

Promoter

Availability

Academic Institutions and Nonprofits only
p072 pCS xGATA4 + xTRPS1(C119)

Plasmid

#11043

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

GATA4 + TRPS1(C119) (trps1, gata4-b Frog)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Fusion construct of XGATA4 (amino acids 1-352) wi…

Promoter

Availability

Academic Institutions and Nonprofits only

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p073 pCS xGATA4tr + xTRPS1(C119)

Plasmid

#11044

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

GATA4tr + TRPS1(C119) (trps1, gata4-b Frog)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Fusion construct of XGATA4 (amino acids 1-274) wi…

Promoter

Availability

Academic Institutions and Nonprofits only

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XE118 CAMKII-T286D-CS2+

Plasmid

#16736

Depositor

Randall Moon

Article

Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

Insert

CAMKII-T286D (Camk2a Rat)

Use

Xenopus expression

Tags

Expression

Mutation

threonine 286 mutated to aspartate

Promoter

Availability

Academic Institutions and Nonprofits only
CaM12(D20A/D56A)/pIRES2-eGFP

Plasmid

#111512

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Use

Tags

Expression

Mammalian

Mutation

D20A/D56A

Promoter

Availability

Academic Institutions and Nonprofits only

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CaM34(D93A/D129A)/pIRES2-eGFP

Plasmid

#111517

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Use

Tags

Expression

Mammalian

Mutation

D93A/D129A

Promoter

Availability

Academic Institutions and Nonprofits only

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CaM1234(D20A/D56AD93A/D129A)/pIRES2-eGFP

Plasmid

#111518

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Use

Tags

Expression

Mammalian

Mutation

D20A/D56AD93A/D129A

Promoter

Availability

Academic Institutions and Nonprofits only

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XE155 XDpr-delta PDZB-CS2+

Plasmid

#16767

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr-delta PDZB (dact1-b Frog)

Use

Xenopus expression

Tags

Expression

Mutation

last four amino acids deleted

Promoter

Availability

Academic Institutions and Nonprofits only
XE161 XDshHA-triple pt mutant-CS2+

Plasmid

#16779

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

Dishevelled (dvl2.L Frog)

Use

Xenopus expression

Tags

HA

Expression

Mutation

triple point mutation outside of the PDZ binding …

Promoter

Availability

Academic Institutions and Nonprofits only