Addgene

We narrowed to 13,506 results for: pET

Showing: 301 - 320 of 13506 results

Results per page:

Sort results by:

pet23a_RBPMS_1_120

Plasmid

#65730

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-120

Promoter

T7

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a.MJ1594

Plasmid

#11413

Depositor

Sung-Hou Kim

Article

Wang et al Structure. 2001 Jan 10. 9(1):65-71.

Insert

phosphoserine phosphatase (MJ_1594 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pet23a_RBPMS_20_101

Plasmid

#65733

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 20-101

Promoter

T7

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pETMutSdelta768

Plasmid

#13041

Depositor

Peggy Hsieh

Article

Biswas et al J Mol Biol. 2001 Jan 26. 305(4):805-16.

Insert

MutS

Use

Tags

Expression

Bacterial

Mutation

deleted C-terminal aa residues 769 - 811

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pET21a.MJ0697

Plasmid

#11411

Depositor

Sung-Hou Kim

Article

Wang et al EMBO J. 2000 Feb 1. 19(3):317-23.

Insert

fibrillarin homolog (MJ_0697 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

M222T

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a.MJ1247

Plasmid

#11414

Depositor

Sung-Hou Kim

Article

Martinez-Cruz et al Structure. 2002 Feb . 10(2):195-204.

Insert

3-hexulose-6-phosphate isomerase (MJ_1247 M. jannaschii)

Use

Tags

T7-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a.MJ0882

Plasmid

#11417

Depositor

Sung-Hou Kim

Article

Huang et al J Struct Funct Genomics. 2002 . 2(3):12

Insert

methyltransferase (MJ_0882 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
CtkA_full_pET21a

Plasmid

#27428

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_300_pET21a

Plasmid

#27429

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

residues 301-325 are truncated

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q_pET21a

Plasmid

#27430

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

D155Q

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D179Q_pET21a

Plasmid

#27431

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

D179Q

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
pET21a.MJ0307

Plasmid

#11415

Depositor

Sung-Hou Kim

Article

Cave et al Protein Sci. 2001 Feb . 10(2):384-96.

Insert

disulfide oxidoreductase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pET21a.TM1363

Plasmid

#11420

Depositor

Sung-Hou Kim

Article

Shin et al J Mol Biol. 2004 Jul 30. 341(1):227-39.

Insert

peptide release factor I (prfA Thermotoga maritima)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
300_pETcon_SARS2_FLip

Plasmid

#222230

Purpose

yeast surface display of the SARS-CoV-2 FLip variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evolution 2024

Insert

SARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
299_pETcon_SARS2_EG-5

Plasmid

#222232

Purpose

yeast surface display of the SARS-CoV-2 EG.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evolution 2024

Insert

SARS-CoV-2 EG.5 RBD (S SARS-CoV-2 virus, Budding Yeast)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_MBP_RBFOX2

Plasmid

#224292

Purpose

MBP-RBFOX2 protein purification from bacteria

Depositor

Chuan He

Article

Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

Insert

RNA binding fox-1 homolog 2 (RBFOX2 Human)

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28Man

Plasmid

#203399

Purpose

exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes

Depositor

David Vocadlo

Article

Deng et al Bioorg Med Chem. 2013 Aug 15;21(16):4839

Insert

man2A

Use

Tags

H6

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pETURALEU

Plasmid

#223207

Purpose

Plasmid for selection/counterslection in budding yeast, containing URA3 and LEU2 markers

Depositor

Sahand Jamal Rahi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_PA4114_Cterm_His

Plasmid

#220484

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_PA1472_Cterm_His

Plasmid

#220485

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge