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pet28a_RBPMS_1_100

Plasmid

#65735

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-100

Promoter

T7

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-N

Plasmid

#156469

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-N (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-M

Plasmid

#156470

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-M (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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ELF4_pet28a

Plasmid

#131661

Purpose

Bacterial expression of ETS gene ELF4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF4 (ELF4 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-YC

Plasmid

#107072

Purpose

Host vector to construct a YPer or CyPet fusion protein

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

YPet-CyPet fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28CLY8

Plasmid

#21767

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 8 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pET24a_UDP_Xase_E113

Plasmid

#73159

Purpose

Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E113A

Depositor

Sandra Gabelli

Article

Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

Insert

pET24a

Use

Tags

Expression

Bacterial

Mutation

change glutamate 113 to alanine

Promoter

Available since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pET11b_MutT

Plasmid

#73155

Purpose

Expresses in E. coli, E coli MutT

Depositor

Sandra Gabelli, Maurice Bessman

Article

Bessman et al J Biol Chem. 1996 Oct 11;271(41):25059-6

Insert

MutT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pet23a_RBPMS_1_120

Plasmid

#65730

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-120

Promoter

T7

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a.MJ1594

Plasmid

#11413

Depositor

Sung-Hou Kim

Article

Wang et al Structure. 2001 Jan 10. 9(1):65-71.

Insert

phosphoserine phosphatase (MJ_1594 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pet23a_RBPMS_20_101

Plasmid

#65733

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 20-101

Promoter

T7

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pETMutSdelta768

Plasmid

#13041

Depositor

Peggy Hsieh

Article

Biswas et al J Mol Biol. 2001 Jan 26. 305(4):805-16.

Insert

MutS

Use

Tags

Expression

Bacterial

Mutation

deleted C-terminal aa residues 769 - 811

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pET21a.MJ0697

Plasmid

#11411

Depositor

Sung-Hou Kim

Article

Wang et al EMBO J. 2000 Feb 1. 19(3):317-23.

Insert

fibrillarin homolog (MJ_0697 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

M222T

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a.MJ1247

Plasmid

#11414

Depositor

Sung-Hou Kim

Article

Martinez-Cruz et al Structure. 2002 Feb . 10(2):195-204.

Insert

3-hexulose-6-phosphate isomerase (MJ_1247 M. jannaschii)

Use

Tags

T7-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a.MJ0882

Plasmid

#11417

Depositor

Sung-Hou Kim

Article

Huang et al J Struct Funct Genomics. 2002 . 2(3):12

Insert

methyltransferase (MJ_0882 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
CtkA_full_pET21a

Plasmid

#27428

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_300_pET21a

Plasmid

#27429

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

residues 301-325 are truncated

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q_pET21a

Plasmid

#27430

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

D155Q

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D179Q_pET21a

Plasmid

#27431

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

D179Q

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
pET21a.MJ0307

Plasmid

#11415

Depositor

Sung-Hou Kim

Article

Cave et al Protein Sci. 2001 Feb . 10(2):384-96.

Insert

disulfide oxidoreductase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only