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Plasmid#184637PurposeFluorescent reporter for mammalian cells: Expression of mScarlet with a CMV 5'UTRDepositorInsertmScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-SspB-mScarlet-I-MYPT169
Plasmid#178522PurposeExpresses SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cellsDepositorInsertSspB-mScarlet-I-MYPT169
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-H2B-V5-mScarlet
Plasmid#191093PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertH2B and mScarlet
UseAAVTagsH2B-V5-mScarletExpressionMammalianMutationThe fusion protein was optimized to the Human cod…PromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mScarlet-H2BC11
Plasmid#207756PurposeDonor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorArticleInsertH2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPOTv6-puro-3Myc:mScarletI:3Myc
Plasmid#179804PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet-I
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV-Camk2-Ace-7aa-mScarlet
Plasmid#129701PurposeExpresses Ace-7aa-mScarlet in mammalian cellsDepositorInsertAce-7aa-mScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterCamk2Available sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mScarlet-Blast
Plasmid#169221PurposeTargeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locusDepositorInsertDouble SapI flanked mScarlet-P2A-Blast
UseTargeting vector backboneTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP mScarlet-MOSPD2 W201E
Plasmid#186477PurposeExpression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsmScarletExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mScarlet-Puro
Plasmid#169220PurposeTargeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locusDepositorInsertDouble SapI flanked mScarlet-P2A-Puro
UseTargeting vector backboneTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-Q63L_mScarlet-i_SGFP2
Plasmid#85072PurposeGreen/red FRET biosensor for RhoA in the GTP locked constitutively active stateDepositorInsertRhoA Biosensor constitutively active (Q63L) (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mScarlet-ACTB
Plasmid#207754PurposeDonor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRISPaint-mScarlet-BlastR [M1G]
Plasmid#171814PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]DepositorInsertmScarlet-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MCP-VPR-2AmScarlet
Plasmid#121964PurposeMammalian expression, Transcription regulation moduleDepositorInsertVP64-p65-Rta fusion module fused with MCP, recruited by MS2 hairpin
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-mScarlet-ACTB
Plasmid#207753PurposeDonor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-mScarlet-Puro-H2BC11
Plasmid#207761PurposeDonor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorArticleInsertH2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pN1-OPA1(123Nt)-mScarletI
Plasmid#129343PurposeMitochondrial intermembrane space localizationDepositorInsertmScarlet(i) (Addgene 85044)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen (pKBJ010)
Plasmid#173461PurposeC-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-Dync1h1motor
Plasmid#191330PurposeExpresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
UseTagsExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644PromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV-Camk2-Ace-8aa-mScarlet
Plasmid#129702PurposeExpresses Ace-8aa-mScarlet in mammalian cellsDepositorInsertAce-8aa-mScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterCamk2Available sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAIDFA-CMV-NmScarlet-mAID
Plasmid#140616PurposeExpression of OsTIR1F74A and mScarlet-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1 mutated F74A
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only