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Plasmid#181909PurposeSingle guide RNA with 2XMS2 loops targeting bacterial lac operatorDepositorInsertsgRNA-2XMS2-lacO
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-2xMS2-mSAT
Plasmid#181908PurposeSingle guide RNA with 2XMS2 loops targeting mouse major satellite repeatsDepositorInsertsgRNA-2XMS2-mSAT
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mHP1alpha
Plasmid#181900PurposeFluorescently tagged HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mHP1gamma
Plasmid#181902PurposeFluorescently tagged HP1gammaDepositorInsertmHP1gamma (Cbx3 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-GFP-VPR
Plasmid#181907PurposeFluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-RtaDepositorInsertdCas9-VPR
UseCRISPRTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMECP2-GFP
Plasmid#181904PurposeFluorescently tagged MECP2, GFPDepositorInsertMECP2 (Mecp2 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH
Plasmid#182425PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteinsDepositorInsertsUseTagsVesicle Nucleating peptide (VNp) and Vesicle Nucl…ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hTRF1
Plasmid#103802PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hLaminB1
Plasmid#103799PurposeBLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-mCherry-DMDEx23-eGFP
Plasmid#211366PurposePiggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)DepositorInsertmCherry-DMDEx23-eGFP
UseTagsExpressionMammalianMutationmCherry interrupted by mdx dystrophin exon 23 bet…PromoterCAGAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hLaminB1
Plasmid#103800PurposeBLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hTRF1
Plasmid#103801PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-mHP1alpha
Plasmid#181911PurposeBacterial protein expression vector for HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET16-mHP1alpha
Plasmid#181910PurposeBacterial protein expression vector for HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-LacI-tagRFP-T
Plasmid#103810Purpose'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMECP2-RFP
Plasmid#181905PurposeFluorescently tagged MECP2, RFPDepositorInsertMECP2 (Mecp2 Mouse)
UseTagsRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-GFP-mHP1alpha
Plasmid#181912PurposeBacterial protein expression vector for GFP-tagged HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsGFPExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald
Plasmid#164210PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmEmeraldExpressionMutationPromoterEF1aAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-PGK-LAMP1-EYFP
Plasmid#164215PurposepLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK promoterDepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsEYFPExpressionMutationPromoterPGKAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-LAMP1-HaloTag
Plasmid#164209PurposeExpresses HaloTag tagged LAMP1 under CMV promoterDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only