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Plasmid#188881PurposeGateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2)DepositorInsertCas9
UseGateway entry vectorTagsExpressionMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
CcCDP_p15A
Plasmid#179272PurposeExpresses CcCDP in E. coli BL21(DE3)DepositorInsertcellodextrin phosphorylase
UseSynthetic BiologyTags6xHISExpressionBacterialMutationPromoterT7 lacOAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 S389,398 A
Plasmid#129485PurposeExpression in S. cerevisiaeDepositorInsertGlo3 S389,398 A (GLO3 Budding Yeast)
UseTagsExpressionYeastMutationMutation of serines 389 and 398 to alaninePromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 S389,398 D
Plasmid#129486PurposeExpression in S. cerevisiaeDepositorInsertGlo3 S389,398 D (GLO3 Budding Yeast)
UseTagsExpressionYeastMutationMutation of serines 389 and 398 to AspPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Met25 Glo3 ΔGRM-ΔC
Plasmid#129487PurposeExpression in S. cerevisiaeDepositorInsertGlo3 ΔGRM ΔC (GLO3 Budding Yeast)
UseTagsExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-GAP-BoCCS
Plasmid#123287PurposeExpression of truncated Glo3 (1-375) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation1-375PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-BoCCS-GRM
Plasmid#123288PurposeExpression of truncated Glo3 (137-459) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation137-459PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-Par-BoCCS-GRM
Plasmid#123289PurposeExpression of truncated Glo3 (296-459) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation296-459PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-BoCCS
Plasmid#123290PurposeExpression of truncated Glo3 (137-375) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation137-375PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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p415 Glo3 R59K ΔC
Plasmid#112653PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K ΔC
UseTagsExpressionYeastMutationR59K, Δ431-493 (deletion of C-terminal amphipathi…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K ΔGRM-ΔC
Plasmid#112654PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K ΔGRM ΔC
UseTagsExpressionYeastMutationR59K, Δ376-493 (deletion of Glo3 motif & C-te…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K S389,398 A
Plasmid#112656PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K S389,398A
UseTagsExpressionYeastMutationR59K, mutations (S389A, S398A) in Glo3 motif, non…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K S389,398 D
Plasmid#112657PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K S389,398D
UseTagsExpressionYeastMutationR59K, mutations (S389D, S398D) in Glo3 motif, pho…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 ΔGRM-ΔC
Plasmid#112659PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 ΔGRM ΔC
UseTagssfGFPExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 A
Plasmid#112660PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 S389,398A
UseTagssfGFPExpressionYeastMutationmutations (S389A, S398A) in Glo3 motif, non-phosp…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 D
Plasmid#112661PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 S389,398D
UseTagssfGFPExpressionYeastMutationmutations (S389D, S398D) in Glo3 motif, phosphomi…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K L246D
Plasmid#112648PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K L246D
UseTagsExpressionYeastMutationR54K & L246D (in ALPS motif)PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K AxxA
Plasmid#112649PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K AxxA
UseTagsExpressionYeastMutationR54K & Alanine substitution of C-terminal δL …PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 Δ3xF AxxA
Plasmid#112650PurposeExpression S.cerevisiaeDepositorInsertGcs1 Δ3xF AxxA
UseTagsExpressionYeastMutationR54K, W304A, W307A, W310A, W349A, F353APromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only