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Plasmid#65563PurposeIntron targeting-mediated Gal4 knockin at the zebrafish th locus.DepositorInsertth-P2A-Gal4 (th Zebrafish)
UseZebrafish injectionTagsExpressionMutationPromoternoneAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-mEOS3.2-TRF2 HR Donor
Plasmid#86845PurposeHR Donor for N-terminal HA-mEOS3.2-TRF2 fusion at endogenous TRF2 locus in human cells.DepositorInsertHA-mEOS3.2-TRF2 at endogenous locus (TERF2 Human)
UseTagsHA-mEOS3.2ExpressionMammalianMutationPromoterEndogenous TRF2 promoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS29: pDonor(PmcCAST)
Plasmid#168162PurposePmcCAST donor harboring non-functional cargo.DepositorInsertsPmcCAST right end (RE)
PmcCAST left end (LE)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97312PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.DepositorInsertActb HMEJ donor
UseLentiviral and Mouse TargetingTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
gfap-EGFP donor
Plasmid#65564PurposeIntron targeting-mediated EGFP knockin at the zebrafish gfap locusDepositorInsertgfap-EGFP (gfap Zebrafish)
UseZebrafish injectionTagsExpressionMutationPromoternoneAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL0747 (pDonor_L-MmeI)
Plasmid#130647PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (L*)
UseCRISPR; TransposonTagsExpressionBacterialMutationTGTTGATG --> TGTTGGAGPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0746 (pDonor_R-MmeI)
Plasmid#130646PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (R*)
UseCRISPR; TransposonTagsExpressionBacterialMutationTGTTGATA --> TGTTGGAAPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330 _HITI donor B6
Plasmid#172847PurposeDRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Id2-Halo donor
Plasmid#113116PurposeDonor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequenceDepositorInsertId2 donor region including homology arms and HaloTag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Donor/Reporter-COL4A5
Plasmid#130280PurposeCOL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor templateDepositorInsertmCherry-eGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV_Sox2 HMEJ donor
Plasmid#97322PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Sox2. AAV backbone.DepositorInsertSox2 HMEJ donor
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
V7 pDNR Dual - Donor
Plasmid#11689DepositorTypeEmpty backboneUseCre/Lox; Donor vectorTagsExpressionMutationPromoterAvailable sinceMay 24, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97310PurposeNHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.DepositorInsertActb NHEJ donor
UseAAV and Mouse TargetingTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV_Nanog HMEJ donor
Plasmid#97321PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.DepositorInsertNanog HMEJ donor
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL0721 (pDonor_L1-105)
Plasmid#130645PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.DepositorInsertVchCAST donor DNA (short L)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV_Cdx2 HMEJ donor
Plasmid#97319PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV backbone.DepositorInsertCdx2 HMEJ donor
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTLR-donor-UAS
Plasmid#119001PurposeRepair template for the TLR reporter with 10 UAS binding sites for yeast Gal4DepositorInsertmVenus
UseTagsExpressionMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTLR-donor-tetO
Plasmid#119000PurposeRepair template for the TLR reporter with 7 tet operator elementsDepositorInsertmVenus
UseTagsExpressionMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV_Dbh HMEJ donor
Plasmid#97320PurposeHMEJ donor for fusing a p2A-mCherry-WPRE reporter to mouse Dbh. AAV backbone.DepositorInsertDbh HMEJ donor
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
mGDF8 donor mCherry-PA
Plasmid#72596PurposePlasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.DepositorInsertmCherry-puromycin cassetter
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only