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iLID-tdTomato-Omp25

Plasmid

#214400

Purpose

Expresses fusion of iLID with tdTomato and transmembrane domain of Omp25

Depositor

Liting Duan

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121

Insert

iLID-tdTomato-Omp25 (Synj2bp Rat, Synthetic)

Use

Tags

iLID-TdTomato-Omp25

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-Cas9-tdTomato

Plasmid

#141013

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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p2301y-tdtomato-dCas9_DegronDead_TPLN188

Plasmid

#205409

Purpose

dCas9 guided repressor

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

dCas9_DegronDead_TPLN188

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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LoxP2-Mb2Tomato-2A (SO240)

Plasmid

#99614

Purpose

To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2Tomato

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-ytdTomato-CaURA3

Plasmid

#118461

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-C–dSpCas9

Plasmid

#157836

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pME tdTomato-NLS

Plasmid

#135205

Purpose

Red fluorescent tag

Depositor

David Tobin

Insert

tdTomato

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_Ras

Plasmid

#209709

Purpose

Mammalian expression of y-ATOM biosensor for HRas

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_Ras

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:Chronos-tdTomato

Plasmid

#124232

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

Chronos-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTIGER_tdTomato::3xFLAG::dCrk

Plasmid

#131138

Purpose

UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Use

Tags

3XFLAG and tdTomato

Expression

Insect

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pX459-sg-tdTomato90/816

Plasmid

#179918

Purpose

Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pXD042 dTomato KIKO

Plasmid

#192154

Purpose

dTomato KIKO

Depositor

Sidi Chen

Article

Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

Insert

dTomato

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-tdTomato

Plasmid

#153424

Purpose

Retroviral (MSCV) expression of tdTomato fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

tdTomato

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pWalium-ChtVis-Tomato

Plasmid

#67756

Purpose

Expresses a fluorescent chitin reporter under Gal4 control in Drosophila.

Depositor

Paul Adler

Article

Sobala et al Development. 2015 Nov 15;142(22):3974-81

Insert

dyl - chitin binding domain - tdTomato - dyl

Use

Tags

td-tomato

Expression

Insect

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAdx-CMV-tdTomato

Plasmid

#73347

Purpose

Express tdTomato under CMV promoter

Depositor

Kazuhiro Oka

Insert

CMV-tdTomato

Use

Adenoviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMUT2-pAto-mCherry

Plasmid

#192858

Purpose

pMUT2 based vector for sensing acetoacetate in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Acetoacetate inducible promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pSCIP-hCD69-TdTomato

Plasmid

#154095

Purpose

Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Inserts

[5HA]-P2A-tdTomato-[3HA]
hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCS-foxc1a-2a-nlstdTomato

Plasmid

#132970

Purpose

full-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

tdTomato
foxc1a (foxc1a Zebrafish)

Use

Tags

NLS and myc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Syn-ChR-tdTomato

Plasmid

#99843

Purpose

AAV expression of ChR2(H134R)-tdTomato under the Syn promoter

Depositor

Gordon Shepherd

Insert

ChR2(H134R)-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

ChR2(H134R), carrying a single point mutation at …

Promoter

human synapsin I promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST tdTomato Rab11 WT

Plasmid

#53473

Depositor

Matthew Scott

Insert

Rab11 (Rab11 Fly)

Use

Tags

tdtomato

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only