Showing: 301 - 320 of 2919 results
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pFL His-I3 V41A / W43A
Plasmid#113515PurposeExpression of His-I3 V41A / W43 A in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*HisExpressionInsectMutationV41A /W43APromoterAvailabilityAcademic Institutions and Nonprofits only -
pFL His-mEos3.2-I3
Plasmid#113516PurposeExpression of His-Eos-I3 in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*His-mEos3.2ExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
deltaLoop-MIDAS
Plasmid#171605PurposeExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.DepositorInsertMIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
UseTagsHis6 tag and SNAP tagExpressionBacterialMutationReplaced amino acids 4458-4496 with ASGSGS linkerPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET15b p97-Strep-His E314Amb
Plasmid#169021PurposeExpresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteriaDepositorInsertp97
UseTagsHis and SBPExpressionBacterialMutationE314TAGPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET15b p97-His D592Amb
Plasmid#169022PurposeExpresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteriaDepositorInsertp97
UseTagsHisExpressionBacterialMutationD592TAGPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 68CVQ
Plasmid#13691DepositorInsertHPV 16 E7 CVQ6870AAA (E7 Human)
UseTagsExpressionMammalianMutationaa 68-70 changed from CVQ to AAAPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCC335
Plasmid#110166PurposeminiMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)DepositorInsertERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationPhospho-acceptor sites mutated: S43A, T55A, T62A.…Promoterlin-31 promoter/enhancerAvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37delta2-81
Plasmid#113502PurposeBacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81DepositorInsertp37 (Ubxn2b Mouse)
UseTagsGST-PreScission protease siteExpressionBacterialMutationdelta 2-81PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 SHPmut
Plasmid#113503PurposeBacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP boxDepositorInsertp37 (Ubxn2b Mouse)
UseTagsGST-PreScission protease siteExpressionBacterialMutationF215A/E218A/QKL220-222AAAPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 deltaUBX
Plasmid#113504PurposeBacterial expression of GST-tagged p37 lacking the UBX domainDepositorInsertp37 (Ubxn2b Mouse)
UseTagsGST-PreScission protease siteExpressionBacterialMutationdelta 251-331 (stop codon behind AA250)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pFL His-SDS22 / PP1gamma
Plasmid#113511PurposeExpression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)DepositorUseTags6*HisExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1
Plasmid#175049PurposeOverexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA
Plasmid#175050PurposeOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D
Plasmid#175051PurposeOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationchanged Thr76 to Asp, phosphomimetic mutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
Tet-off TauRD (P301L/V337M)
Plasmid#188572PurposeDox-repressible expression of the Tau repeat domainDepositorInsertTau repeat domain (MAPT Human)
UseLentiviralTagsMycExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailabilityAcademic Institutions and Nonprofits only -
Tet-off Full length Tau (0N4R, P301L/V337M)
Plasmid#188573PurposeDox-repressible expression of the 0N4R Tau isoformDepositorInsert0N4R Tau (MAPT Human)
UseLentiviralTagsExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailabilityAcademic Institutions and Nonprofits only -
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APP_Halo_C_allele
Plasmid#178137PurposeDonor vector for endogenous tagging of human APP at the C-terminus with halotagInsertHalotag (APP Human)
UseDonor vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPwt-T2A-mCherry
Plasmid#190803PurposeExpress wild-type APP from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPP (APP Human)
UseLentiviralTagsExpressionMutationPromoterUbi CAvailabilityAcademic Institutions and Nonprofits only
Showing: 301 - 320 of 2919 results