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pQC_HA-p115 TEV-Myc #77#407

Plasmid

#42127

Depositor

Ayano Satoh

Article

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Insert

p115

Use

Retroviral

Tags

HA and Myc

Expression

Mammalian

Mutation

inserted ENLYFQ b/w 886 and 887

Promoter

CMV

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-THAP11(AA132-313)

Plasmid

#37002

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11(AA132-313) (THAP11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acids 132 to 313

Promoter

Available since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pBABE-puro-THAP11-rescue-3xFLAG

Plasmid

#36998

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 (THAP11 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Mutations were generated at codons 273-275 conver…

Promoter

Available since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pTRIP TRE-BI GFP10-RHOB/RBD-GFP11

Plasmid

#182231

Purpose

Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Inserts

ras homolog family member B (RHOB Human)
rhotekin RHO binding domain (RBD)

Use

Lentiviral

Tags

GFP10 (split-GFP tripartite) and GFP11 (M4)

Expression

Mutation

Promoter

TRE bidirectional

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

Plasmid

#86413

Purpose

nuclear split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

Nop1pr-GFP11-mCherry-PUS1

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)

Plasmid

#86416

Purpose

ONM/ER split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP11-mCherry-Scs2TM

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

Plasmid

#86417

Purpose

luminal split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-mCherry-SCS2TM-GFP11

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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ATF4 2: Mouse ATF4 (CHOP11/cATF)-bZIP

Plasmid

#21849

Depositor

David Ron

Insert

ATF4 (Atf4 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

N-terminal transactivation domain deleted and rep…

Promoter

Available since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)

Plasmid

#106464

Purpose

Expresses Prc1 with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

PRC1

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

PRC1

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE-BI GFP10-RHOA/RBD-GFP11

Plasmid

#182232

Purpose

Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Inserts

ras homolog family member A (RHOA Human)
rhotekin RHO binding domain (RBD)

Use

Lentiviral

Tags

GFP10 (split-GFP tripartite) and GFP11 (M4)

Expression

Mutation

Promoter

TRE bidirectional

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKP115 (pRS406-YBR139Wp-YBR139W-GFP-ADH1t)

Plasmid

#106465

Purpose

Expresses Atg42/Ybr139w with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

Plasmid

#211468

Purpose

Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

4xGFP11-SEC61B

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2

Plasmid

#180359

Purpose

mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN 9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2

Plasmid

#180361

Purpose

mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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NSP11-1x-BioID2 pBabe-puro (SARS-CoV-2)

Plasmid

#186005

Purpose

Expresses SARS-CoV-2 NSP11 fused to BioID2 and HA tag

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 NSP11 (ORF1ab SARS-CoV-2)

Use

Retroviral

Tags

BioID2 and HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

Plasmid

#106462

Purpose

Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(N163,242Q)

Use

Tags

PA

Expression

Bacterial and Yeast

Mutation

changed Asparagines at positions 163 and 242 to G…

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_HA-p115-TEV-cleaved #957#382

Plasmid

#42107

Depositor

Ayano Satoh

Article

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Insert

p115

Use

Tags

HA

Expression

Mammalian

Mutation

deleted 886 to end and added ENLYFQ

Promoter

CMV

Available since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

Plasmid

#164038

Purpose

Expression of dCas9-14xGFP11 in mammalian cells

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

dCas9-14xGFP11

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

dSV40

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11

Plasmid

#162037

Purpose

Background-free mRNA labeling tripartite superfolder GFP system

Depositor

Hye Yoon Park

Article

Park et al RNA. 2020 Jan;26(1):101-109. doi: 10.126

Insert

GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10

Plasmid

#180357

Purpose

mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN 2 (SEPTIN2 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT2 F20D, V27D

Promoter

bidirectional TRE

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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