-
Plasmid#206417PurposeMammalian expression of hGBF1DepositorInsertGBF1 (GBF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-CtIP-siR-WT
Plasmid#82030PurposePlasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertCtIP (RBBP8 Human)
UseTagsHAExpressionMammalianMutationMutations making the cDNA resistant to a siRNAPromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICE-EGFP-FLAG-Ku70siR-Mut6E
Plasmid#46962PurposeFor constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.DepositorInsertKu70 (XRCC6 Human)
UseTagsGFP-FLAGExpressionMammalianMutationMut6E corresponding to K282E K287E T300E K331E K3…PromoterCMV TetAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40
Plasmid#180758PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)DepositorInsertα-COPI-WD40
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Leu181Lys, Leu…PromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(T308A)
Plasmid#208088PurposeMammalian expression of the human LTB4 receptor LTB4R with the T308A mutation.DepositorInsertLTB4R(T308A) (LTB4R Human)
UseLentiviralTagsExpressionMammalianMutationT308APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(S310A)
Plasmid#208089PurposeMammalian expression of the human LTB4 receptor LTB4R with the S310A mutation.DepositorInsertLTB4R(S310A) (LTB4R Human)
UseLentiviralTagsExpressionMammalianMutationS310APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(T308A,S310A)
Plasmid#208090PurposeMammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations.DepositorInsertLTB4R(T308A,S310A) (LTB4R Human)
UseLentiviralTagsExpressionMammalianMutationT308A, S310APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_3xHA-LTB4R
Plasmid#208091PurposeMammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.DepositorInsert3xHA-LTB4R (LTB4R Human)
UseLentiviralTags3xHAExpressionMammalianMutationnonePromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Xpress-GBF1(E794K)
Plasmid#206418PurposeMammalian expression of hGBF1(E794K)DepositorInsertGBF1 (GBF1 Human)
UseTagsExpressionMammalianMutationE794KPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb no wild type
Plasmid#158205PurposeCassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.DepositorInsert10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONRG_P4-P1R:TBA2p
Plasmid#128429PurposeGateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.DepositorInsertTESTA ABUNDANT 2 (AT1G62060 Mustard Weed)
UseGateway promoter entry cloneTagsExpressionMutationPromoterTESTA ABUNDANT 2Available sinceOct. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRf1-4CL5-AtSDT (JBEI-11535)
Plasmid#87934PurposeCo-expression in S.cerevisiae of 4CL5 and AtSDTDepositorUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Myc-tagged PSK
Plasmid#197149PurposeExpression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cellsDepositorInsertTAOK2 (TAOK2 Human)
UseTagsMycExpressionMammalianMutationWild TypePromoterSV40Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP
Plasmid#170327PurposeDouble-floxed inverse Orientation HA and IRES-YFP tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-rTetO-EGFP-T2A-RPL22-3xHA
Plasmid#170326PurposeTetracycline-inducible expression of HA and EGFP tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-TK-5UTR-BsmBI-Luciferase
Plasmid#114670PurposeFor one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporterDepositorInsertLacZ
UseLuciferaseTagsfor cloning 5' UTR-Luciferase fusionsExpressionMammalianMutationPromoterlac promoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Synaptojanin 1-170
Plasmid#22292DepositorInsertSynaptojanin 1_170 (SYNJ1 Human)
UseTagsFlagExpressionBacterial and MammalianMutationK334R compared to Genbank ID NM_003895PromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro
Plasmid#170317PurposeExpresses HA and EGFP tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo
Plasmid#170319PurposeExpresses HA and EGFP tagged RPL22, neomycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro
Plasmid#170318PurposeExpresses FLAG and EGFP tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only