Skip to main content
Addgene
Showing: 301 - 320 of 437 results

  1. pChlr-5SE

    Plasmid
    #52160
    Purpose
    Encodes module 5 with amino acids 12S-16E for recognition of nt 4G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16E in Module 5
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pChlr-5NQ

    Plasmid
    #52161
    Purpose
    Encodes module 5 with amino acids 12N-16Q for recognition of nt 4U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12C to 12N in Module 5
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pChlr-6CQ

    Plasmid
    #52163
    Purpose
    Encodes module 6 with amino acids 12C-16Q for recognition of nt 3A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in Module 6
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pChlr-6SR

    Plasmid
    #52166
    Purpose
    Encodes module 6 with amino acids 12S-16R for recognition of nt 3C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 6
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pChlr-4NQ

    Plasmid
    #52153
    Purpose
    Encodes module 4 with amino acids 12N-16Q for recognition of nt 5U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    52153-plasmid-map-sequence-id-80994

    Enlarge

  6. pChlr-5CQ

    Plasmid
    #52159
    Purpose
    Encodes module 5 with amino acids 12C-16Q for recognition of nt 4A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    52159-plasmid-map-sequence-id-80995

    Enlarge

  7. pChlr-6NQ

    Plasmid
    #52165
    Purpose
    Encodes module 6 with amino acids 12N-16Q for recognition of nt 3U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    52165-plasmid-map-sequence-id-80996

    Enlarge

  8. pChlr-8NQ

    Plasmid
    #52173
    Purpose
    Encodes module 8 with amino acids 12N-16Q for recognition of nt 1U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    52173-plasmid-map-sequence-id-80998

    Enlarge

  9. pU6_(GLuc)_INT[GFPApt]

    Plasmid
    #68428
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing the GFP aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68428-plasmid-map-sequence-id-120206

    Enlarge

  10. pU6_(GLuc)_INT[3xK-T]

    Plasmid
    #68429
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three kink-turns
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68429-plasmid-map-sequence-id-125557

    Enlarge

  11. pU6_(GLuc)_INT[S1Apt]

    Plasmid
    #68425
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct with S1 streptavidin aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68425-plasmid-map-sequence-id-120203

    Enlarge

  14. pU6_(GLuc)_INT[BoBS]

    Plasmid
    #68431
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68431-plasmid-map-sequence-id-125558

    Enlarge

  15. pU6_(GLuc)_INT[3xPP7_SL]

    Plasmid
    #68424
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68424-plasmid-map-sequence-id-125555

    Enlarge

  16. pU6_(GLuc)_INT[3xMS2_SL]

    Plasmid
    #68426
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three MS2 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68426-plasmid-map-sequence-id-125556

    Enlarge

  17. pUBC_V5_MS2-mVenus

    Plasmid
    #107606
    Purpose
    Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    MS2-mVenus
    Use
    Synthetic Biology
    Tags
    V5 and mVenus
    Expression
    Mutation
    MS2: V75E/A81G
    Promoter
    human UBC
    Available since
    May 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107606-plasmid-map-sequence-id-209713

    Enlarge

  18. pCMV/3' Box_(GLuc)_INT

    Plasmid
    #68436
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    68436-plasmid-map-sequence-id-120214

    Enlarge

  19. pUBC_HA_PP7-mCherry

    Plasmid
    #107605
    Purpose
    Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    PP7-mCherry
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    hUBC
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    107605-plasmid-map-sequence-id-207484

    Enlarge

  20. pCMV/MASC_(GLuc)_INT

    Plasmid
    #68440
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    68440-plasmid-map-sequence-id-124660

    Enlarge

Showing: 301 - 320 of 437 results