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pMIA10.7
Plasmid#214451PurposeBxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.DepositorInsertsTet-On 3G
PuroR
TRE3G CopGFP
UseBxbi donorTagsPuroR, T2A, and Tet-On 3GExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-Sumo
Plasmid#106980PurposeLR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tagDepositorTypeEmpty backboneUseTagsHis8-SumoExpressionBacterialMutationPromoterT7Available sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRB051_LEU2
Plasmid#196993PurposeBig-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAR4-CD4d3+4-bio
Plasmid#73118PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding NAR4 with rat CD4d3+4-bioDepositorInsertNAR4 (ART4 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_GFP
Plasmid#78172PurposeDoxycyline regulated expression of GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dom34(full length)
Plasmid#19098DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Rv2416c_wild
Plasmid#37649DepositorInsertRv2416c (eis Mycobacterium tuberculosis)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
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Dom34(1-138)
Plasmid#19101DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34(265-386)
Plasmid#19102DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tag and N tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34(265-377)
Plasmid#19103DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tag and N tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Tip alpha K102A/K104A (21-192)
Plasmid#23159DepositorInsertTNF-α-inducing protein
UseTagsHisExpressionBacterialMutationchanged lysine 102 and lysine 104 to alanine, and…PromoterAvailable sinceApril 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
Dom34 K82A
Plasmid#19112DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationK82APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
SSO0083
Plasmid#19097DepositorInsertPelA (Cell division protein pelota homolog) (Orf c04039 protein)
UseTagsN tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSAD-5PSD95-EGFP-SynPhRFP
Plasmid#217979PurposeG-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFPDepositorUseG-deleted rabiesTagsEGFP and RFPExpressionMutationPromoterNoneAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG431
Plasmid#165615PurposeVector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
UseCRISPRTagsSV40 NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ta1098 E140A
Plasmid#19085DepositorInsertCell division protein pelota related protein (TA_RS05685 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationE140APromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34(1-377)
Plasmid#19100DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only