Showing: 301 - 320 of 403 results
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Plasmid#45355DepositorInsertDHFR Ub Mek Ura3
UseTagsExpressionYeastMutationPromoterpCUP1AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pPL5618_pUG_MtxR
Plasmid#60929PurposePCR template vector for generating methotrexate resistance cassette.DepositorInsertDihydrofolate reductase (DHFR Human)
UseLoxp flanked deletion cassette templateTagsExpressionMutationL22F F31SPromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-PEST
Plasmid#204766PurposeGEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)DepositorInsertEGFP-PEST-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
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pILGFP8EFA
Plasmid#185854PurposeTesting a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationDHFR P66LPromoterAvailabilityAcademic Institutions and Nonprofits only -
CAG-DDdCas9VP192-T2A-EGFP-ires-puro
Plasmid#69534PurposeDHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.DepositorInsertDD-dCas9VP192-T2A-EGFP
UseTagsC-term T2A-EGFP and DHFR Destabilised DomainExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal.Sth.dCas9
Plasmid#171088PurposeCRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.DepositorInsertsToxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
DHFR-TSc3
UseToxoplasma gondii expressionTagsExpressionMutationNote: A S245F mutation is present but did not app…PromoterDHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…AvailabilityAcademic Institutions and Nonprofits only -
pMTL5
Plasmid#127967PurposeDHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pCyCEN_Lisp2mCherry_hsp70_GFP
Plasmid#137169PurposeDual fluorescent reporter for liver stage expression in Plasmodium cynomolgiDepositorInsertsmCherry
Nanoluc
dihydrofolate reductase
Green Fluorescent Protein
UseUnspecifiedTagsT2AExpressionMutationPromoterP. cynomolgi hsp70 and P. cynomolgi lisp2AvailabilityAcademic Institutions and Nonprofits only -
pDonorTp
Plasmid#211788PurposepDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpRDepositorInsertdhfr
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
WT_S1-H1
Plasmid#218392Purpose3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intronDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailabilityAcademic Institutions and Nonprofits only -
WT_S7-H1
Plasmid#218393Purpose3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intronDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailabilityAcademic Institutions and Nonprofits only -
MUT_N-H1
Plasmid#218397Purpose3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-N
Plasmid#218398Purpose3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailabilityAcademic Institutions and Nonprofits only -
pCB268
Plasmid#53363PurposepcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.DepositorUseTags13xMyc, Flag, and HAExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-H1
Plasmid#218394Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailabilityAcademic Institutions and Nonprofits only -
MUT_S7-H1
Plasmid#218396Purpose3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailabilityAcademic Institutions and Nonprofits only -
pSL1423
Plasmid#194904PurposepSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for Plasmodium falciparumDepositorInsertTurboID
UsePlasmodium falciparumTagsGFPmut2ExpressionMutationPromoterNone. This allows a C-terminal fusion to a protei…AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-spacer-H1
Plasmid#218399Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 301 - 320 of 403 results