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tracrRNA(scr)-CJ8421_04975 mRNA

Plasmid

#199718

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and scrambled tracrRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Hic1 GFP

Plasmid

#106953

Purpose

Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

anti-Hic1 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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L1_Cas9-Ck4

Plasmid

#136135

Purpose

L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpEF1a:Cas9_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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PlmdCas12e KRAB

Plasmid

#188506

Purpose

Expresses FLAG tagged PlmdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-KRAB-NLS

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSC32

Plasmid

#104806

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from AtUBQ10 promoter.

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101090

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC31

Plasmid

#104805

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101090 (Pen3). Also expresses Cas9 from Gmubi promoter.

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101090

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC27

Plasmid

#104801

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr4g020620 (Pho2ab). Also expresses Cas9 from Gmubi promoter.

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr4g020620

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC26

Plasmid

#104800

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101040 (Mel1). Also expresses Cas9 from AtUBQ10 promoter.

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101040

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC20

Plasmid

#104794

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from AtUBQ10 promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g020630

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSC14

Plasmid

#104788

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from AtUBQ10 promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g044580

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSC15

Plasmid

#104789

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from rolD promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g044580

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC13

Plasmid

#104787

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from Gmubi promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g044580

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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AWP-031

Plasmid

#213967

Purpose

Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.

Depositor

Alexander Westermann

Article

Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

Insert

Cas12a (cas12a Synthetic, Segatella bryantii)

Use

Tags

3XFLAG

Expression

Bacterial

Mutation

D875A to inactivate target nuclease activity

Promoter

PcfxA, IPTG inducible

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSC39

Plasmid

#104813

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101180 (Cop-like). Also expresses Cas9 from Gmubi promoter.

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101180

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC34

Plasmid

#104808

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr8g060730 (Fbl1-like). Also expresses Cas9 from AtUBQ10 promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr8g060730

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC33

Plasmid

#104807

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr8g060730 (Fbl1-like). Also expresses Cas9 from Gmubi promoter.

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr8g060730

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC25

Plasmid

#104799

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101040 (Mel1). Also expresses Cas9 from Gmubi promoter from Gmubi promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101040

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC24

Plasmid

#104798

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre2). Also expresses Cas9 from rolD promoter from AtUBQ10 promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101120, Medtr2g101130

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC22

Plasmid

#104796

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre1). Also expresses Cas9 from Gmubi promoter from AtUBQ10 promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101120, Medtr2g101130

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC19

Plasmid

#104793

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from Gmubi promoter from Gmubi promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g020630

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge