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pMINTC3H

Plasmid

#110075

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pOBCol3.6-GFPtpz

Plasmid

#110211

Purpose

Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPtopaz

Use

Tags

None

Expression

Mammalian

Mutation

None

Promoter

3.6 kb rat type 1 collagen + first intron

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Hyg)

Plasmid

#110077

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCOND (Apr)

Plasmid

#110094

Purpose

Suicide vector based on the TetR/Pip OFF system.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBb(B5)8k-sfGFP

Plasmid

#110140

Purpose

IPTG-inducible pTRC promoter expressing sfGFP on BBR1-B5 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMEXC3GH (Hyg)

Plasmid

#110082

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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p043 mTcf-4 B

Plasmid

#11031

Depositor

Ramesh Shivdasani

Article

Lee et al J Biol Chem. 1999 Jan 15. 274(3):1566-72

Insert

Tcf-4 (Tcf7l2 Mouse)

Use

Xenopus, avian, and zebrafish expression

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
Hy_pMT Flag-Hel25E WT

Plasmid

#110123

Purpose

Expresses Flag-tagged D. melanogaster Hel25E

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Oct. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E K91N

Plasmid

#110121

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Use

Tags

Flag

Expression

Insect

Mutation

K91N mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E D193E

Plasmid

#110120

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Use

Tags

Flag

Expression

Insect

Mutation

D193E mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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KG#65

Plasmid

#110933

Purpose

Expresses proteins in cholinergic neurons in the ventral nerve cord of C. elegans

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

Inserts

unc-17 beta promoter
unc-54 3' control region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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tet-pLKO.puro_shGAC

Plasmid

#110423

Purpose

shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1/TO (RNA PolIII)

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMEXC3GH (Kan)

Plasmid

#110081

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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Human USP30 (64-502, construct 2, MG-26-18)

Plasmid

#110744

Purpose

Bacterial expression of human USP30 (construct 2)

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Use

Tags

N-His6-3C-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_EIF4E_IF4E

Plasmid

#110010

Purpose

Protein expression and purification of EIF4E_IF4E

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EIF4E_IF4E (EIF4E Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-MyosinVb-Ctail

Plasmid

#110170

Purpose

Dominant negative effect by competing with MyosinVb C-terminal tail interactions

Depositor

Jose Esteban

Article

Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

Insert

Myosin Vb (Myo5b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 1262-1670

Promoter

CMV

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AURKB_Pkinase

Plasmid

#110025

Purpose

Protein expression and purification of AURKB_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AURKB_Pkinase (AURKB Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CHD1 (CHD1A-c107)

Plasmid

#110253

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

CHD1 (CHD1 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7 - lacO

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pQC hGAB.wt V5-His IRES G418

Plasmid

#110333

Purpose

Mammalian retroviral expression of glutaminase 2 isoform GAB (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

Insert

GLS2 Glutaminase 2 (GLS2 Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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SOAP-COMP-blac-flag-his

Plasmid

#110997

Purpose

Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

Depositor

Gavin Wright

Article

Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

Insert

secreted ookinete adhesive protein (SOAP) (PF3D7_1404300 Synthetic)

Use

Tags

Exogenous signal peptide of mouse origin and rat …

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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