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GFP-PH-FAPP1

Plasmid

#161986

Purpose

Expresses GFP-tagged PH-FAPP1. PI(4)P biosensor

Depositor

Tamas Balla, David Bryant

Article

Balla et al Mol Biol Cell. 2005 Mar;16(3):1282-95. d

Insert

PH-FAPP1 (PLEKHA3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C-SLC35B2

Plasmid

#155037

Purpose

Transient expression of EGFP-SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLz26_CsoS2_N1-N2_GFP

Plasmid

#140851

Purpose

His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeats 1-2 fused to GFP

Use

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa

Plasmid

#127389

Purpose

Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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CD8-GFP-FKBP

Plasmid

#100751

Purpose

Expression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

CD8A (CD8A Human)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX303 GFP-PIK3C2A

Plasmid

#162000

Purpose

Lentiviral expression vector containing PIK3C2A tagged with GFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2A (PIK3C2A Human)

Use

Lentiviral

Tags

eGFP

Expression

Mutation

Promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pUAST ɑSynWT-EGFP

Plasmid

#201249

Purpose

allows the expression of the insert in Drosophila melanogaster

Depositor

Pedro Domingos

Article

Pocas et al Hum Mol Genet. 2015 Apr 1;24(7):1898-907

Insert

ɑ-Synuclein (SNCA Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FRB-VMAc-CGFP

Plasmid

#70226

Purpose

Expresses C-terminal portion of GFP along with VMA intein for protein splicing

Depositor

Perry Howard

Article

Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

Insert

eGFP

Use

Tags

FKBP-rapamycin binding (FRB) domain of the mammal…

Expression

Mammalian

Mutation

Changed Ile 129 to Cys, Deleted amino acids 1-128

Promoter

CMV

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P41nmt1-mEGFP

Plasmid

#105140

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P41nmt1-mEGFP

Use

Tags

41nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mEGFP

Plasmid

#105137

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P3nmt1-mEGFP

Use

Tags

3nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mEGFP

Plasmid

#105143

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P81nmt1-mEGFP

Use

Tags

81nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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α‐eGFP

Plasmid

#160214

Purpose

Retroviral construct to express an eGFP-tagged version of the α subunit of AP2

Depositor

Sandra Schmid

Article

Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10

Insert

α‐eGFP (Ap2a2, Ap2a1 Mouse)

Use

Retroviral

Tags

eGFP at Hinge region

Expression

Mutation

Brain-specific Insert

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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LSS-SGFP2-aTub

Plasmid

#112936

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Insert

alpha tubulin

Use

Tags

LSS-SGFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pATZ-HA-GFP11

Plasmid

#177720

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec24D

Plasmid

#32678

Depositor

Henry Lester

Article

Richards et al J Biol Chem. 2011 Sep 9;286(36):31241-9.

Insert

Sec24D (SEC24D Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

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pAT-WT-HA-GFP11

Plasmid

#177719

Purpose

ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pssGFP-hCRT

Plasmid

#80022

Purpose

Mammalian expression of signal peptide-EGFP-humanCRT

Depositor

Chinten James Lim

Article

Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi

Insert

CRT (CALR Human)

Use

Tags

EGFP and signal peptide

Expression

Mammalian

Mutation

signal peptide 17aa added before GFP-CRT, will ta…

Promoter

CMV

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pCS2+ Zf GFP-vclb

Plasmid

#185411

Purpose

GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin b (vclb Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf GFP-vcla

Plasmid

#185410

Purpose

GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin a (vcla Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-hs-AKT1-PH-EGFP-6H

Plasmid

#187157

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000142208 (AKT1 Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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