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p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167848

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Use

Tags

6xHis and AviTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET tdTomato LIC cloning vector (u-tdTomato)

Plasmid

#86926

Purpose

pET LIC cloning vector for yORF-tdTomato

Depositor

Chris Jeans

Type

Empty backbone

Use

Tags

tdTomato

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

Plasmid

#166796

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

KK93EE

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

Plasmid

#166800

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

E597R

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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mHDAC7-Unspliced (23-938) pEF6-Flag (MJS_018)

Plasmid

#73269

Purpose

mHDAC7-U (23-938) pEF6-V5 (MJS_017) (Flag tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 (Pelican)

Plasmid

#212401

Purpose

Encodes SARS-CoV-2 spike D614G,Q677P for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D614G,Q677P (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

D614G,Q677P

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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gWiz_BM40-vAchT-Cytosolic-VH-hIgG1-CH1-TS-HIS

Plasmid

#218446

Purpose

mammalian expression plasmid for the heavy chain fragment of an anti-vAchT Fab domain binding to the cytosolic side of the transporter

Depositor

Axel Brunger

Insert

vAchT-Cytosolic-Vh-hIgG1-CH6

Use

Affinity Reagent/ Antibody

Tags

BM40 and TwinStrep-6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G

Plasmid

#169960

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG and Ub

Expression

Mammalian

Mutation

F370G

Promoter

CMV

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A

Plasmid

#169955

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S297A, R394E

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A

Plasmid

#169957

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S297A, F405G

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368G

Plasmid

#169959

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG and Ub

Expression

Mammalian

Mutation

L368G

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394E

Plasmid

#169961

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG and Ub

Expression

Mammalian

Mutation

R394E

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405G

Plasmid

#170119

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG and Ub

Expression

Mammalian

Mutation

F405G

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A

Plasmid

#169951

Purpose

Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E274R, S297A

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE

Plasmid

#166805

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The LL1193E mutant abolishes FL-NLRP1 association with DPP9 at interface I.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

LL193EE

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG K1277E

Plasmid

#166808

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K1277E

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG H1276G

Plasmid

#166807

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H1276G

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S RK96EE

Plasmid

#166797

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. RK96EE mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

RK96EE

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL102EE

Plasmid

#166799

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. LL102EE mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

LL102EE

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S D808N

Plasmid

#166802

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. The D808N mutant is catalytically dead.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

D808N

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 301 - 320 of 11892 results

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