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pMSCV MEKK3 PB1

Plasmid

#12185

Depositor

Gary Johnson

Article

Nakamura et al J Biol Chem. 2003 Sep 26. 278(39):36989-

Insert

MEKK3 (Map3k3 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

PB1 domain: aa 36-127

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
p667-UBC-MAP3K14-V5-miniTurbo_IDG-K

Plasmid

#189903

Purpose

Gateway destination clone of MAP3K14 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

MAP3K14 (MAP3K14 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSicoR human Drosha2

Plasmid

#14767

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

Drosha shRNA (DROSHA Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pMSCV mouse Hic-5

Plasmid

#11190

Depositor

Bruce Spiegelman

Article

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Insert

Hic-5 (Tgfb1i1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pAc-sgRNA-Cas9.scr_2

Plasmid

#190704

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ERN2_IDG-K

Plasmid

#189905

Purpose

Gateway destination clone of ERN2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

ERN2 (ERN2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSuper mouse DGCR8-1

Plasmid

#14772

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

DGCR8 shRNA (Dgcr8 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pLV.PARP1#5

Plasmid

#14548

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

PARP1 shRNA (PARP1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2007

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv1.mir451_KO

Plasmid

#164689

Purpose

For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-451 CRISPR KO gRNA (MIR451A )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shGFP

Plasmid

#83085

Purpose

Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

Plasmid

#164800

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA1
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pWXLd-RIEP-OgNLuc

Plasmid

#135936

Purpose

Expresses the OgNLuc LumiFluor in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalB wt

Plasmid

#19720

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog B (RALB Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pMRXIP SECFP-Stx17TM

Plasmid

#86777

Purpose

Expresses transmembrane domains of syntaxin17 tagged with SECFP in mammalian cells

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

transmembrane domains of human Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

SECFP

Expression

Mutation

Promoter

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLV.PARP1#4

Plasmid

#14547

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

PARP1 shRNA (PARP1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1-shRalA-1

Plasmid

#15257

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

v-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shLacZ

Plasmid

#98632

Purpose

Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shLacZ

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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CRISPR_TRBC

Plasmid

#164994

Purpose

Expression of gRNA targeting TCRbeta constant locus

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against TRBC

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dIDR1)

Plasmid

#192809

Purpose

Stable expression of 3xFLAH-NCOA4 (delta IDR1) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the IDR1

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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