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p5xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180477PurposeInducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInserts5x QUAS-ZsGreen-P2A
mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSJ982
Plasmid#176622PurposeExpress yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertUb-MPGLW-Fbp1
UseTagsUbiquitin N-terminal fusion and triple flag tag C…ExpressionYeastMutationChanged first 4 N-terminal amino acid residues of…PromoterTDH3tc3Available sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-3xFLAG-Puro PAK5 K478R/479R S364L
Plasmid#167199Purposepuromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutantDepositorInsertPAK5 K478R/479R S364L
UseLentiviralTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlinkG2-PTPIP51(236-470)
Plasmid#170534PurposeExpresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsGST tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(236-470)
Plasmid#170530PurposeExpresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)
UseTagsHis tagExpressionBacterialMutationPromoterT5 promoterAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-3xFLAG-Puro PAK5 K478R/479R D421N
Plasmid#167200Purposepuromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutantDepositorInsertPAK5 K478R/479R D421N
UseLentiviralTagsExpressionMammalianMutationPromoterUBCAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-3xFLAG-Puro PAK5 K478R/479R E294K
Plasmid#167198Purposepuromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutantDepositorInsertPAK5 K478R/479R E294K
UseLentiviralTagsExpressionMammalianMutationPromoterUBCAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-3xFLAG-Puro PAK5 K478R/479R M173I
Plasmid#167197Purposepuromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutantDepositorInsertPAK5 K478R/479R M173I
UseLentiviralTagsExpressionMammalianMutationPromoterUBCAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-3xFLAG-Puro PAK5 K478R/479R E144K
Plasmid#167196Purposepuromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutantDepositorInsertPAK5 K478R/479R E144K
UseLentiviralTagsExpressionMammalianMutationPromoterUBCAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
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VP2C-GFP-MIOX only
Plasmid#166684PurposeYeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertVP2C-GFP-MIOX
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 only
Plasmid#166675PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertMurine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 MUT
Plasmid#110126PurposeExpresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activityDepositorInsertURH49 (DDX39A Human)
UseTagsFlagExpressionInsectMutation4 amino acid mutations (RSFS motif changed to KSL…PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E MUT
Plasmid#110122PurposeExpresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activityDepositorInsertHel25E (Hel25E Fly)
UseTagsFlagExpressionInsectMutation4 mutations (KKLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM33
Plasmid#154167PurposeAedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertRel2 (long isoform)
UseTagsFLAGExpressionInsectMutationC-terminal IκB domain deletedPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S159A
Plasmid#131520PurposeExpresses MKP-3 with S159A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Serine 159 to AlaninePromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR152
Plasmid#106052PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR152; Opto-GPR152; E6)DepositorInsertOpto-GPR152 (GPR152 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR176
Plasmid#106060PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR176; Opto-GPR176; F2)DepositorInsertOpto-GPR176 (GPR176 Human, Bovine)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only
