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Plasmid#129293PurposeGateway entry clone encoding human ATG3 C264A (catalytic inactive)DepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationchanged Cysteine 264 to AlaninePromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ATG3 allKR
Plasmid#129295PurposeGateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginineDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationchanged all 22 Lysine residues to ArgininePromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 allKR
Plasmid#129298PurposeExpresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged all 22 Lysine residues to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 1-13 KR
Plasmid#129299PurposeExpresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged first 13 Lysine residues to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 14-22 KR
Plasmid#129300PurposeExpresses pCMV 3xFLAG-ATG3 14-22 KR (last nine lysines mutated to arginine) in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged last 9 Lysine residues to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 K295R
Plasmid#129302PurposeExpresses pCMV 3xFLAG-ATG3 K295R in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged Lysine 295 to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824E-S828E
Plasmid#91999PurposeExpresses FLAG-HA-AGO2 (S824E-S828E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824E, S828EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824E-S831E
Plasmid#92000PurposeExpresses FLAG-HA-AGO2 (S824E-S831E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824E, S831EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S828E-S831E
Plasmid#92001PurposeExpresses FLAG-HA-AGO2 (S828E-S831E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS828E, S831EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824E-S828E-S831E
Plasmid#92002PurposeExpresses FLAG-HA-AGO2 (S824E-S828E-S831E)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824E, S828E, S831EPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-S824A-T830A
Plasmid#92003PurposeExpresses FLAG-HA-AGO2 (S824A-T830A)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824A, T830APromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHISSSO6206
Plasmid#97065PurposeExpressing Sulfolobus solfataricus orf SSO6206 proteinDepositorInsertS. solfataricus orf SSO6206
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hBcl10_#16
Plasmid#75253Purposeretroviral shRNA vector against human BCL10, expresses GFP for transduction controlDepositorInsertshRNA against hBcl10 (BCL10 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hBcl10_#2
Plasmid#75254Purposeretroviral shRNA vector against human BCL10, expresses GFP for transduction controlDepositorInsertshRNA against hBcl10 (BCL10 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_hBcl10_#1
Plasmid#75248Purposeretroviral shRNA vector against human BCL10, expresses GFP for transduction controlDepositorInsertshRNA against hBcl10 (BCL10 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromotermU6Available sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Dnmt3b6-Poly(A)-NeoR
Plasmid#65552PurposePlasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationisoform 6PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP
Plasmid#102634PurposeIn vitro expression of sfGFP from the T7 promoterDepositorInsertsfGFP
UseTagsstrep tagExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FRA1
Plasmid#188665PurposeExpresses murine FRA1 (Fosl1)DepositorInsertFosl1 (Fosl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterhPGKAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0663 ACTB atgRNA with v2 scaffold
Plasmid#179108PurposeACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffoldDepositorInsertACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0209 ACTB Nicking guide +48
Plasmid#179109PurposeACTB N-term Nicking guide 1 +48 guideDepositorInsertACTB N-term Nicking guide 1 +48 guide
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only