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Plasmid#141183PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-FcDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
UseTagsFc region of IgG1 (a.a. Asp-221 to Lys-447) and S…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2(WT)-Fc(IgG1)
Plasmid#145163PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTBL210
Plasmid#126451PurposeExpresses Cas9-XTEN-TdT in mammalian cells.DepositorInsertCas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)
UseTagsExpressionMammalianMutationSee depositor comments belowPromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-R1441C
Plasmid#25046DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationArginine 1441 mutated to CysteinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps
Plasmid#181956PurposeGenetically encoded FRET-based cAMP indicator fused to AKAP79.DepositorInsertAKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
UseTagsAKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MET-V5/HIS
Plasmid#201982Purposeexpression of human MET receptor tyrosine kinase in mammalian cellsDepositorInsertMET receptor tyrosine kinase (MET Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-Y1699C
Plasmid#25048DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationTyrosine 1699 mutated to CysteinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-cDAXX-0SB
Plasmid#169728PurposeMammalian expression construct of GFP tagged cDAXX-0SBDepositorInsertcDAXX-0SB (DAXX Human)
UseTagsGFPExpressionMammalianMutationComprises residues 495-741 and has the major SB m…PromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2(WT)-Fc(IgA1)
Plasmid#145154PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgA1)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgA1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y
Plasmid#164845PurposeMammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tagDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
UseTags8his tag and HA leader sequenceExpressionMammalianMutationCodon-optimized for human cell expression. Has N5…PromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-ScAct1*-thymosinB-8His
Plasmid#111148PurposeExpresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37DepositorInsertAct1 (ACT1 Budding Yeast)
UsePichia pastoris integration plasmidTagsThymosin beta and a His-tagExpressionYeastMutationPromoterAOX1Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTBL469
Plasmid#126458PurposeExpresses Cas9-15xFlag-TdT in mammalian cells.DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-His-Max-A-PQBP1-Y65C
Plasmid#25803DepositorInsertPQBP1 with Y65C mutation (PQBP1 Human)
UseTagsHis and XpressExpressionMammalianMutationTyrosine 65 within the WW domain of PQBP1 is chan…PromoterAvailable sinceJuly 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TYRO3-V5/HIS
Plasmid#200118PurposeExpression of human TYRO3 receptor tyrosine kinase in mammalian cellsDepositorInserthuman TYRO3 receptor tyrosine kinase (TYRO3 Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-K1347A
Plasmid#25052DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationLysine 1347 mutated to AlaninePromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-ROC
Plasmid#25053DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationContains only the ROC domain- aa 1333-1516PromoterAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-CXCR4-VN
Plasmid#98964PurposeFLAG-tagged human CXCR4 fused to N-terminus of split VenusDepositorInsertCXCR4 (CXCR4 Human)
UseTagsFLAG (dykdddd) epitope tag, Signal/leader sequenc…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-CXCR4-VC
Plasmid#98967Purposemyc-tagged human CXCR4 fused to C-terminus of split VenusDepositorInsertCXCR4 (CXCR4 Human)
UseTagsSignal/leader sequence from Influenza A virus HA …ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HLA-c-myc-optiT1R3 ECD-MHC
Plasmid#113961Purposemammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonicalDepositorInsertT1R3 (TAS1R3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationresidues 21-563 fused to a transmembrane tetherPromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL472
Plasmid#126457PurposeExpresses Cas9-10xFlag-TdT in mammalian cells.DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only