Addgene

We narrowed to 6,795 results for: 11

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MTK3_010

Plasmid

#123725

Purpose

Encodes Tet-On 3G transactivator protein as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TetOn3G

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pB-Neo-GFP-hMyo19 iso2

Plasmid

#210333

Purpose

Overexpression of hMyo19

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

hMyo19 (MYO19 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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EYFP-hDF-deltaTM-pCMV-9

Plasmid

#72586

Purpose

human dysferlin no transmembrane domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

transmembrane domain deleted

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMis1_1B

Plasmid

#192079

Purpose

A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

Depositor

Markus Buchhaupt

Article

Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

Inserts

KanR
rhaR
rhaS
pBBR1 rep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

Plasmid

#174892

Purpose

amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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shCDK4/6(2)

Plasmid

#73555

Purpose

vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4 + shRNA against CDK6

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
CFP-CD82

Plasmid

#1818

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

CD82 (CD82 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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PVT1-luciferase

Plasmid

#113350

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_PA4114_Cterm_His

Plasmid

#220484

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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FL tension sensor module

Plasmid

#101170

Purpose

Expresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.

Depositor

Carsten Grashoff

Article

Ringer et al Nat Methods. 2017 Nov;14(11):1090-1096.

Insert

YPet(short)-FL-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3_V5-APEX2-Sec61B

Plasmid

#83412

Purpose

Expressed N-term tagged APEX2 on Sec61B in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

V5-APEX2-Sec61B (SEC61B Human, Synthetic)

Use

Tags

APEX2 and V5

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX NBP1 (P#415)

Plasmid

#14658

Depositor

Matija Peterlin

Article

Lu et al Immunity. 1998 May . 8(5):647-56.

Insert

ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pCDHblast MCSNard OST-LMNA

Plasmid

#22661

Depositor

Tom Misteli

Article

Pegoraro et al Nat Cell Biol. 2009 Oct . 11(10):1261-7.

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

Onestrep Tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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PVT1-Tol2

Plasmid

#113381

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Tol2 reporter

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

Plasmid

#174891

Purpose

amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIP TBRII

Plasmid

#19147

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

Transforming growth factor, beta receptor II (TGFBR2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
His-Keap1-Tev-Halo

Plasmid

#87545

Purpose

expresses C-terminal Halo-tagged human Keap1 in mammalian cells

Depositor

Yimon Aye

Article

Parvez et al Nat Protoc. 2016 Dec;11(12):2328-2356. d

Insert

Keap1 (KEAP1 Human)

Use

Tags

Halo and His-Tag

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-CRY2(D387A)-mCherry

Plasmid

#58369

Purpose

Light insensitive CRY2 mutant

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (D387A) (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

D387A

Promoter

CMV

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pKD077

Plasmid

#136473

Purpose

SSB-mTur2 IDL fusion expression plasmid

Depositor

James Keck

Article

Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

Insert

SSB-mTur2 (ssb E. coli)

Use

Tags

mTurquoise2

Expression

Bacterial

Mutation

mTurquoise2 inserted between Phe148 and Ser149

Promoter

T7 promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMIY-mETV3-Flag

Plasmid

#34579

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Use

Retroviral

Tags

FLAG and IRES-YFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only