Addgene

We narrowed to 3,798 results for: SPL

Showing: 3141 - 3160 of 3798 results

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SNAP-EF(N)-5nmSAH-GFP(1-10)

Plasmid

#61659

Purpose

5nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-5nmSAH-GFP(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAPM sh1 DNMT1

Plasmid

#88884

Purpose

Lentiviral vector expressing shRNA against murine DNMT1

Depositor

Silvia Monticelli

Article

Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

Insert

shRNA no.1 DNMT1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV (spleen focus forming virus)

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2ALexA

Plasmid

#125222

Purpose

Artificial phase 2 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

LexA

Use

Other

Tags

T2A

Expression

Mutation

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Use

Tags

Expression

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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D5 gene in PETCON vector

Plasmid

#87626

Purpose

expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

Depositor

Sarel Fleishman

Article

Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

Insert

Designed allosteric Zn2+ and fluorescein binding antibody scFv D5

Use

Tags

3x(G4S) Linker and cMyc tag on backbone

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+MT-hSeparase

Plasmid

#33017

Depositor

Marc Kirschner

Article

Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

Insert

Separase (ESPL1 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
SNAP-EF(N)-30nmSAH-GFP(1-10)

Plasmid

#61662

Purpose

30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-30nmSAH-GFP(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCZGY3195

Plasmid

#172695

Purpose

Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

Punc-25

Use

Tags

GFP 1-10

Expression

Worm

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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TOPO Venus

Plasmid

#68493

Purpose

TOPO construct expressing Venus for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

split Venus

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pJin-239

Plasmid

#125716

Purpose

ABA-inducible split T7 gRNA for GFP vector using RNAPN N-29-1

Depositor

Bryan Dickinson

Article

Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

Insert

pT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAG144 SLC23A2 3'UTR

Plasmid

#12002

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N9 3'UTR (SLC23A2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pWS936

Plasmid

#123050

Purpose

G_ subunit for creating C-terminal transplants

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 C-terminal Truncation Vector [3-4a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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Human NCKX4-AS-short (pcDNA3.1+)

Plasmid

#75210

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Use

Tags

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJAT22

Plasmid

#204311

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-GFPn

Use

CRISPR

Tags

Expression

Mutation

Promoter

3XP3

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-T-Lg

Plasmid

#216977

Purpose

Expression of GvpT-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpT-Linker-Large BiT

Use

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Sm-U

Plasmid

#217003

Purpose

Expression of Small BiT-Linker-GvpU

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Small BiT-Linker-GvpU

Use

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-G-Sm

Plasmid

#216990

Purpose

Expression of GvpG-Linker-Small BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpG-Linker-Small BiT

Use

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-N-Lg

Plasmid

#216963

Purpose

Expression of GvpN-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpN-Linker-Large BiT

Use

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-T-Sm

Plasmid

#217000

Purpose

Expression of GvpT-Linker-Small BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpT-Linker-Small BiT

Use

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Sm-J

Plasmid

#216999

Purpose

Expression of Small BiT-Linker-GvpJ

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Small BiT-Linker-GvpJ

Use

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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