Addgene

We narrowed to 13,516 results for: pET

Showing: 3121 - 3140 of 13516 results

Results per page:

Sort results by:

pET15b/GVcl 1-1066 /*TBM

Plasmid

#162781

Purpose

amplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain II

Depositor

Laura Lafon-Hughes

Article

et al PeerJ

Insert

Gallus gallus vinculin (VCL Chicken)

Use

Tags

Expression

Bacterial

Mutation

changed Gly 454 to Val

Promoter

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Pet22b-mEGFP-YAP-C-IDR

Plasmid

#166441

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-C-IDR(266-504) (YAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b-rShHTL7 cPGFP(E167)

Plasmid

#166004

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(E167)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b-rShHTL7 cPGFP(D165)

Plasmid

#166003

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(D165)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b-rShHTL7 cPGFP(C164)

Plasmid

#166002

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(C164)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b-DAD2 cPGFP(N180)

Plasmid

#166000

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(N180)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b-DAD2 cPGFP(A165)

Plasmid

#165999

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(A165)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b-HisDAD2 cPGFP(G164)

Plasmid

#165997

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

HisDAD2 cPGFP(G164)

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b-rShHTL7 cPGFP(L166)

Plasmid

#165995

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(L166)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b-DAD2 cPGFP(G164)

Plasmid

#165993

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(G164)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM10 with rat Δ192Annexin A11

Plasmid

#164498

Purpose

For bacterial expression of rat His-tagged Δ192Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ192Annexin A11 (Anxa11 Rat)

Use

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM10 with rat Δ188Annexin A11

Plasmid

#164497

Purpose

For bacterial expression of rat His-tagged Δ188Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ188Annexin A11 (Anxa11 Rat)

Use

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CPH1141 (pET151_Spt6 1247-1451)

Plasmid

#91815

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

deleted residues 1-1246

Promoter

T7

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-YPET-C1-WPRE

Plasmid

#159954

Purpose

untargeted marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

YPET-C1

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a RUVBL2 Delete

Plasmid

#159143

Purpose

Expression and protien purification of a mutated RUVBL2

Depositor

Eric Erquan Zhang

Article

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL2 (RUVBL2 Human)

Use

Tags

Expression

Bacterial

Mutation

amino acid 2-456, Thr127-Glu233 mutated to GPPG

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15-His-∆NGlpG(Y205F)

Plasmid

#155132

Purpose

Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Use

Tags

His

Expression

Bacterial

Mutation

Y205F, insert codes for GlpG residues 87-276

Promoter

T7

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDUET-GST AO7RE Y165A

Plasmid

#139428

Purpose

GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Changed tyrosine 165 to an alanine

Promoter

T7 promoter-1

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDUET-GST AO7RE P199A

Plasmid

#139427

Purpose

GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Changed proline 199 to an alanine

Promoter

T7 promoter-1

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDUET-GST AO7RE I137A

Plasmid

#139319

Purpose

GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Changed isoleucine 137 to an alanine

Promoter

T7 promoter-1

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-FLAG-SpCas9-HF1

Plasmid

#126770

Purpose

Expression of increased fidelity SpCas9-HF1 in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge