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Plasmid#227118PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0405DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0405 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0408-luc2
Plasmid#227120PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0408DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0408 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0441-luc2
Plasmid#227129PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0441DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0441 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-BC0498-luc2
Plasmid#227134PurposeBarcoded assay, cAMP/Ca2+ sensor; barcode BC0498DepositorInsert6x clustered CRE element linked to CMV minimal promoter driving barcode 0498 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0440-luc2
Plasmid#227128PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0440DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0440 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMVmin-BC0524-luc2
Plasmid#227139PurposeBarcoded assay, Ca2+ sensor; barcode BC0524DepositorInsert6x clustered NFAT element linked to CMV minimal promoter driving barcode 0524 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-BC0504-luc2
Plasmid#227135PurposeBarcoded assay, cAMP/Ca2+ sensor; barcode BC0504DepositorInsert6x clustered CRE element linked to CMV minimal promoter driving barcode 0504 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0442-luc2
Plasmid#227130PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0442DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0442 and a luciferase reporter gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-shNC-mCherry
Plasmid#222962PurposeNegative control for lentiviral shRNADepositorInsertNegative control
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD80-CLIP
Plasmid#223590PurposeLentiviral expression of human CD80 with CLIP tag in mammalian cellsDepositorInsertCD80 (CD80 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-CD80
Plasmid#223588PurposeLentiviral expression of human CD80 with SNAP tag in mammalian cellsDepositorInsertCD80 (CD80 Human)
UseLentiviral; mammalian expressionTagsSNAPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-SPNS1-sg1
Plasmid#198566PurposeSPNS1-targeting sgRNA in PX458 vectorDepositorInsertSPNS1-sg1 (SPNS1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-SPNS1-sg2
Plasmid#198567PurposeSPNS1-targeting sgRNA in PX458 vectorDepositorInsertSPNS1-sg2 (SPNS1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-IRAK4-mScarlet-P2A-Puro
Plasmid#221237PurposeStable expression of mouse IRAK4-mScarlet after Lentiviral transductionDepositorInsertIRAK4 (Irak4 Mouse)
UseLentiviralTagsmScarletExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-K17R-K22R
Plasmid#220294Purposemammalian expression of Bcl-2-K17R-K22R tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationK17R-K22RPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-G128L-A131L-T132L
Plasmid#220304Purposemammalian expression of Bcl-2-G128L-A131L-T132L tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationG128L-A131L-T132LPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NIP45 wt
Plasmid#210266PurposeInducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 wt siRES (NFATC2IP Synthetic)
UseTagsmCherryExpressionMammalianMutationsiRESPromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 wt
Plasmid#210262PurposeInducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 wt siRES (NFATC2IP Synthetic)
UseTagsEGFPExpressionMammalianMutationsiRESPromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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sgVRK1-hygro
Plasmid#199640PurposesgRNA guide against VRK1DepositorInsertN/A (VRK1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only