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pRetroX-TRE3G_NT-GSDMD_I105N_

Plasmid

#201174

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Tags

Expression

Mutation

aa1-276 only, I105N

Promoter

TRE3G

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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p7136 pHAGE-P-CMVt-N-HA-GAW-EIF3H

Plasmid

#100148

Purpose

Expresses EIF3EH

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

EIF3H (EIF3H Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMB73

Plasmid

#73086

Purpose

Expresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_NoCys-Myc

Plasmid

#201169

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

C39A, C57A, C77A, C122A, C265A, C192A

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2-HA

Plasmid

#133065

Purpose

Retroviral expression of mouse MD2 tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (Ly96 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag2AUF1(p40)

Plasmid

#135945

Purpose

Expresses AUF1p40 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag2AUF1(p42)

Plasmid

#135946

Purpose

Expresses AUF1p42 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMB71

Plasmid

#73085

Purpose

Expresses C. elegans optimized BirA under control of the intestinal elt-2 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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HA-VHL C162F-pRc/CMV

Plasmid

#22042

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant, converting aa 162 from Cysteine to …

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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HA-VHL 1-167-pRc/CMV

Plasmid

#22078

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

deletion mutant covering only aa 1-167 of wt VHL …

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pMB37

Plasmid

#73084

Purpose

Expresses C. elegans optimized BirA under control of the ubiquitous rps-27 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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HA-VHL L158S-pRc/CMV

Plasmid

#22041

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant converting aa 162 from Cysteine to P…

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR3c GW CHD5

Plasmid

#68869

Purpose

GW entry vector for CHD5

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-B-pCR

Plasmid

#46369

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

Insert

calretinin promoter (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

promoter

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
p7137 pHAGE-P-CMVt-N-HA-GAW-EIF3M

Plasmid

#100149

Purpose

Expresses EIF3M

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

EIF3M (EIF3M Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pNTKV- Utf1-KI-3'UTR-2A-tdTomato-RBGpA

Plasmid

#128834

Purpose

Knock-in of tdTomato into mouse Utf1 locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

tdTomato into Utf1 locus (Utf1 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-UBE3BΔHECT-copGFP-Puro

Plasmid

#107343

Purpose

Expresses UBE3B deletion of HECT domain fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B(ΔHECT)-copGFP (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

deletion of HECT domain (757-end)

Promoter

CMV

Available since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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p7055 pHAGE-P-CMVt-N-HA-GAW-COPZ1

Plasmid

#100145

Purpose

Expresses COPZ1

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

COPZ1 (COPZ1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-MCS-shCHD5 #1

Plasmid

#68876

Purpose

CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pLKO-CreERT2 sgRipk4 v3

Plasmid

#158048

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Ripk4 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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